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The influence of endogenous cathepsin in different subcellular fractions on the quality deterioration of Northern pike (Esox lucius) fillets during refrigeration and partial freezing storage

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Abstract

The purpose of this study was to investigate the endogenous cathepsin activity in each subcellular fraction and the effect of this activity on myofibrillar protein and texture during refrigeration and partial freezing storage of northern pike (Esox lucius) fillets. The results showed that fillets stored under the refrigerated condition were more susceptible to oxidation than partial freezing. Endogenous cathepsin activity indicated that partial freezing destroys the integrity of lysosomes more effectively than refrigeration and inhibits the increase in cathepsin B and B + L in lysosomes. The activity of cathepsin B and B + L in lysosomes, mitochondria and myofibrils under the partial freezing conditions was always lower than that under refrigeration. Texture analysis showed that refrigeration had a negative impact on hardness and springiness. In conclusion, the cathepsin activity in each subcellular fraction was effectively inhibited and better textural characteristics were obtained with partial freezing than refrigeration.

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Acknowledgements

We acknowledged the financial support from the National Natural Science Foundation of China (Grant No. 31460438) and the National Natural Science Foundation of China (Grant No. 31960460).

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Authors and Affiliations

  1. Food College, Shihezi University, North Fourth Road, Shihezi, 832003, Xinjiang, China

    Hengheng Qiu, Xin Guo, Xiaorong Deng, Xiaobing Guo, Xiaoying Mao, Chengjian Xu & Jian Zhang

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  1. Hengheng Qiu
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  2. Xin Guo
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  3. Xiaorong Deng
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  4. Xiaobing Guo
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  5. Xiaoying Mao
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  7. Jian Zhang
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Correspondence to Jian Zhang.

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Qiu, H., Guo, X., Deng, X. et al. The influence of endogenous cathepsin in different subcellular fractions on the quality deterioration of Northern pike (Esox lucius) fillets during refrigeration and partial freezing storage. Food Sci Biotechnol 29, 1331–1341 (2020). https://doi.org/10.1007/s10068-020-00781-z

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  • DOI: https://doi.org/10.1007/s10068-020-00781-z

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