Abstract
The purpose of this study is to investigate the effect of fermented ginseng extract by Lactobacillus brevis (FGE) on lipopolysaccharide (LPS)-activated macrophages and 12-O-tetradecanoylphorbol-13-acetate (TPA)-mediated dermatitis in mice. FGE showed better anti-inflammatory activities than ginseng extract on the formation of nitric monooxide, IL-6, TNF-α, and IL-10 within non-cytotoxicity range in LPS-activated RAW264.7 cells. In addition, FGE reduced the expression of cyclooxygenase-2 and inducible nitric oxide synthase through inhibiting nuclear translocation of NF-κB. Consistent with in vitro experiments, FGE dose-dependently suppressed ear edema, and formation of TNF-α and IL-6, and it (50 mg/mL) significantly enhanced IL-10 level in ear tissues of TPA-treated mice. In conclusions, FGE has anti-dermatitic activity through inhibiting the activation of macrophages. Such effects of FGE are associated with suppressing nuclear translocation of NF-κB. Therefore, the features of FGE may provide the information for its application for therapy and prevention of dermatitis.
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Abbreviations
- COX-2:
-
Cyclooxygenase-2
- FGE:
-
Extract of fermented ginseng fine root
- GE:
-
Extract of ginseng fine root
- IL-6:
-
Interleukin-6
- iNOS:
-
Inducible nitric oxide synthase
- LPS:
-
Lipopolysaccharide
- NO:
-
Nitric monoxide
- TLR4:
-
Toll-like receptor 4
- TNF-α:
-
Tumor necrosis factor-α
- TPA:
-
2-O-tetradecanoylphorbol-13-acetate
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Acknowledgements
This work was funded by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (Grant Number 2017R1D1A3B03027867).
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Lee, J.Y., Yoo, JM., Baek, S.Y. et al. Anti-dermatitic effect of fermented ginseng extract including rich compound K through inhibiting activation of macrophage. Food Sci Biotechnol 28, 1845–1852 (2019). https://doi.org/10.1007/s10068-019-00632-6
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DOI: https://doi.org/10.1007/s10068-019-00632-6