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Extraction of caffeine and catechins using microwave-assisted and ultrasonic extraction from green tea leaves: an optimization study by the IV-optimal design

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Abstract

In this research, optimal conditions for extraction of caffeine and polyphenols were established from Iranian green tea leaves. In the first step, caffeine was extracted with efficacy about 86% versed to 4.5% of EGC + EGCG. The EGCG + EGC was extracted from partially decaffeinated green tea leaves through microwave-assisted extraction (MAE) and ultrasound-assisted extraction (USE) with efficiency levels of 95 and 85%, respectively. The best results for the MAE process were obtained with 7.8 min and three number of extraction cycles and for the USE process were as followed: time 57 min, temperature 65 °C, and the number of extraction cycles 3. The total phenol content values at the best conditions of MAE and the USE processes were 125 ± 5 and 96 ± 6 mg gallic acid/g DW. The 50% inhibition (IC50) on 1,1-diphenyl-2-picrylhydrazyl (DPPH) were 56 and 66 mg/g of phenol for the MAE and USE processes.

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References

  1. Hoa C, Chena C, Wanasundara U, Shahidi F. Natural antioxidants from tea. Natural Antioxidants: Chemistry, Health Effects, and Applications: 213–223 (1997)

  2. Finger A, Engelhardt UH, Wray V. Flavonol triglycosides containing galactose in tea. Phytochem. 30: 2057–2060 (1991)

    Article  CAS  Google Scholar 

  3. Watson RR, Preedy VR. Bioactive foods and extracts: Cancer treatment and prevention. CRC Press (2010)

  4. Choung MG, Hwang YS, Lee MS, Lee J, Kang ST, Jun TH. Comparison of extraction and isolation efficiency of catechins and caffeine from green tea leaves using different solvent systems. Int. J. Food Sci. Technol. 49: 1572–1578 (2014)

    Article  CAS  Google Scholar 

  5. Vuong QV, Roach PD. Caffeine in green tea: its removal and isolation. Sep. Purif. Rev. 43: 155–174 (2014)

    Article  CAS  Google Scholar 

  6. Ko M-J, Cheigh C-I, Chung M-S. Optimization of subcritical water extraction of flavanols from green tea leaves. J. Agric. Food. Chem 62: 6828–6833 (2014)

    Article  CAS  Google Scholar 

  7. Gujar J, Wagh S, Gaikar V. Experimental and modeling studies on microwave-assisted extraction of thymol from seeds of Trachyspermum ammi (TA). Sep. Purif. Technol. 70: 257–264 (2010)

    Article  CAS  Google Scholar 

  8. Knorr D, Zenker M, Heinz V, Lee D-U. Applications and potential of ultrasonics in food processing. Trends Food Sci. Technol 15: 261–266 (2004)

    Article  CAS  Google Scholar 

  9. Both S, Chemat F, Strube J. Extraction of polyphenols from black tea–Conventional and ultrasound assisted extraction. Ultrason. Sonochem 21: 1030–1034 (2014)

    Article  CAS  Google Scholar 

  10. Yolmeh M, Najafi MBH, Farhoosh R. Optimisation of ultrasound-assisted extraction of natural pigment from annatto seeds by response surface methodology (RSM). Food Chem. 155: 319–324 (2014)

    Article  CAS  Google Scholar 

  11. Beringhs AO, Dalmina M, Creczynski-Pasa TB, Sonaglio D. Response Surface Methodology IV-Optimal design applied to the performance improvement of an RP-HPLC-UV method for the quantification of phenolic acids in Cecropia glaziovii products. Rev. bras. Farmacogn. 25: 513–521 (2015)

    Article  CAS  Google Scholar 

  12. Labbé D, Tremblay A, Bazinet L. Effect of brewing temperature and duration on green tea catechin solubilization: Basis for production of EGC and EGCG-enriched fractions. Sep. Purif. Technol. 49: 1–9 (2006)

    Article  Google Scholar 

  13. Zuo Y, Chen H, Deng Y. Simultaneous determination of catechins, caffeine and gallic acids in green, Oolong, black and pu-erh teas using HPLC with a photodiode array detector. Talanta 57: 307–316 (2002)

    Article  CAS  Google Scholar 

  14. ISO 14502-1:2005. Determination of substances characteristic of green and black tea. Part 1: content of total polyphenols in tea. Colorimetric method using FolineCiocalteu reagent

  15. ISO 10727:2002. Tea and instant tea in solid form - Determination of caffeine content – Method using high-performance liquid chromatography

  16. ISO 14502-2:2005. Determination of substances characteristic of green and black tea—Part 2: Content of catechins in green tea—Method using high-performance liquid chromatography

  17. Fenglin H, Ruili L, Liang M. Free radical scavenging activity of extracts prepared from fresh leaves of selected Chinese medicinal plants. Fitoterapia 75: 14–23 (2004)

    Article  Google Scholar 

  18. Jones B, Goos P. I-optimal versus D-optimal split-plot response surface designs. J. QUAL. TECHNOL. 44: 85 (2012)

    Google Scholar 

  19. Suteerapataranon S, Butsoongnern J, Punturat P, Jorpalit W, Thanomsilp C. Caffeine in Chiang Rai tea infusions: Effects of tea variety, type, leaf form, and infusion conditions. Food Chem. 114: 1335–1338 (2009)

    Article  CAS  Google Scholar 

  20. Liang H, Liang Y, Dong J, Lu J, Xu H, Wang H. Decaffeination of fresh green tea leaf (Camellia sinensis) by hot water treatment. Food Chem. 101: 1451–1456 (2007)

    Article  CAS  Google Scholar 

  21. Bermejo DV, Luna P, Manic MS, Najdanovic-Visak V, Reglero G, Fornari T. Extraction of caffeine from natural matter using a bio-renewable agrochemical solvent. Food Bioprod. Process. 91: 303–309 (2013)

    Article  CAS  Google Scholar 

  22. Sun Q-L, Hua S, Ye J-H, Lu J-L, Zheng X-Q, Liang Y-R. Decaffeination of green tea by supercritical carbon dioxide. J. Med. Plant Res. 4: 1161–1168 (2010)

    CAS  Google Scholar 

  23. Bermejo DV, Mendiola JA, Ibáñez E, Reglero G, Fornari T. Pressurized liquid extraction of caffeine and catechins from green tea leaves using ethyl lactate, water and ethyl lactate + water mixtures. Food Bioprod. 96: 106–112 (2015)

    Article  CAS  Google Scholar 

  24. Li D-C, Jiang J-G. Optimization of the microwave-assisted extraction conditions of tea polyphenols from green tea. J. Food Sci. Nutr. 61: 837–845 (2010)

    CAS  Google Scholar 

  25. Quan PT, Hang T, Hai Ha N, De NX, Tuyen TN. Microwave-assisted extraction of polyphenols from fresh tea shoots. Sci. & Tech. Develop., 9(8), 69–75

  26. Perva-Uzunalić A, Škerget M, Knez Ž, Weinreich B, Otto F, Grüner S. Extraction of active ingredients from green tea (Camellia sinensis): Extraction efficiency of major catechins and caffeine. Food Chem. 96: 597–605 (2006)

    Article  Google Scholar 

  27. Xiao W, Han L, Shi B. Microwave-assisted extraction of flavonoids from Radix Astragali. Sep. Purif. Technol. 62: 614–618 (2008)

    Article  CAS  Google Scholar 

  28. Chemat F, Rombaut N, Sicaire A-G, Meullemiestre A, Fabiano-Tixier A-S, Abert-Vian M. Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrason. Sonochem. 34: 540–560 (2017)

    Article  CAS  Google Scholar 

  29. Choung M-G, Lee M-S. Optimal extraction conditions for simultaneous determination of catechins and caffeine in green tea leaves. Food Sci. Biotechnol. 20: 327–333 (2011)

    Article  Google Scholar 

  30. Hitoshi K, Nobuyoshi M. Extraction of Catechins from Green Tea Using Ultrasound. Jpn. J. Appl. Phys. 46: 4936 (2007)

    Article  Google Scholar 

  31. Xia T, Shi S, Wan X. Impact of ultrasonic-assisted extraction on the chemical and sensory quality of tea infusion. J. Food Eng. 74: 557–560 (2006)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Food Science and Technology, Faculty of Nutrition Science, Food Science and Technology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Vahid Ghasemzadeh-mohammadi, Bahman Zamani & Abdorreza Mohammadi

  2. Department of Inorganic Chemistry, Chemistry and Chemical Engineering Research Center of Iran, Tehran, Iran

    Maryam Afsharpour

Authors
  1. Vahid Ghasemzadeh-mohammadi
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  2. Bahman Zamani
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  3. Maryam Afsharpour
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  4. Abdorreza Mohammadi
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Correspondence to Abdorreza Mohammadi.

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Ghasemzadeh-mohammadi, V., Zamani, B., Afsharpour, M. et al. Extraction of caffeine and catechins using microwave-assisted and ultrasonic extraction from green tea leaves: an optimization study by the IV-optimal design. Food Sci Biotechnol 26, 1281–1290 (2017). https://doi.org/10.1007/s10068-017-0182-3

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  • DOI: https://doi.org/10.1007/s10068-017-0182-3

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