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Effect of Enterococcus faecalis EF-2001 on experimentally induced atopic eczema in mice

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Abstract

Here, the effects of heat-killed Enterococcus faecalis EF-2001 (EF-2001) on atopic eczema (AE) were assessed. An AE model was established in vivo by repetitious topical exposure to 1-chloro-2,4-dinitrobenzene (CDNB) and dermatophagoidesfarinae extract (DFE) via application on each ear. Mice were administered EF-2001 orally for 4 weeks, dermal and epidermal ear thickness, mast cell infiltration of the ear tissue, and serum IgE and IgG2a levels were evaluated. Moreover, pathogenic cytokines levels of the ears, splenocytes, and cervical lymph nodes were determined. EF-2001 reduced AE symptoms grounded in the ear thickness, histopathological analysis, and serum IgE levels. Furthermore, EF-2001 attenuated mast cell infiltration in the ears and CDNB/DFE-induced various pathogenic cytokines levels of the ears, splenocytes and cervical lymph nodes. Thus, our data suggested that EF-2001 may have potential medicinal applications in the treatment of AE through its immunomodulatory properties.

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Authors and Affiliations

  1. Division of Sport Science, College of Science and Technology, Konkuk University, Chungju, Chungbuk, 27478, Korea

    Eun-Ju Choi

  2. R&D Center, Korea BeRM Co., Ltd., Seoul, 06163, Korea

    Masahiro Iwasa, Kwon-Il Han & Wan-Jae Kim

  3. Division of Food BioScience, College of Biomedical and Health Sciences, Konkuk University, Chungju, Chungbuk, 27478, Korea

    Yujiao Tang & Eun-Kyung Kim

  4. Department of Pathology, Sanbon Medical Center, Wonkwang University, Sanbon, Gyeonggi, 15865, Korea

    Weon Cheol Han

  5. School of Life Sciences, Immune Synapse Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju, 61005, Korea

    Zee-Yong Park

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  1. Eun-Ju Choi
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Correspondence to Zee-Yong Park.

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Choi, EJ., Iwasa, M., Han, KI. et al. Effect of Enterococcus faecalis EF-2001 on experimentally induced atopic eczema in mice. Food Sci Biotechnol 25, 1087–1093 (2016). https://doi.org/10.1007/s10068-016-0175-7

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  • DOI: https://doi.org/10.1007/s10068-016-0175-7

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