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Physiological concentrations of trans-11 18:1 vaccenic acid suppress pro-inflammatory markers under acute inflammation in isolated ICR mice splenocytes

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Abstract

Direct influences of dietary trans-11 18:1 vaccenic acid (TVA) at physiological concentrations of 50-200 μM were evaluated for cell growth, cytotoxic activity, and cytokine production in leukocytes isolated from the mouse spleen. TVA supplementation for 24 h induced growth of splenocytes at concentrations of 50-200 μM, except for 100 μM. The cytokines TNFα, IFNγ, and IL-10 of splenocytes were stimulated by 100 μM TVA. Induced production of TNFα in splenocytes challenged with lipopolisaccharides was suppressed by 100 μM TVA. Physiological levels of TVA had direct effects on growth and cytokine production in splenocytes. Further in vivo studies are needed to improve understanding of the precise influence of trans fatty acids on production of pro-inflammatory markers under acute inflammation conditions.

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Authors and Affiliations

  1. Department of Animal Science and Technology, College of Animal Bioscience & Technology, Konkuk University, Seoul, 05029, Korea

    Jae-Sung Lee, Ji-Na Lim, Sang-Bum Lee, Jin-Hee Hwang, U-Suk Jung, Min-Jeong Kim & Hong-Gu Lee

  2. College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China

    Tao Wang

  3. Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, 28644, Korea

    Seong-Ho Choi

  4. Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-0808, Japan

    Satoshi Ishizuka

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  1. Jae-Sung Lee
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  2. Ji-Na Lim
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  3. Tao Wang
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  4. Sang-Bum Lee
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  5. Jin-Hee Hwang
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  7. Min-Jeong Kim
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  9. Satoshi Ishizuka
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  10. Hong-Gu Lee
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Correspondence to Hong-Gu Lee.

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Lee, JS., Lim, JN., Wang, T. et al. Physiological concentrations of trans-11 18:1 vaccenic acid suppress pro-inflammatory markers under acute inflammation in isolated ICR mice splenocytes. Food Sci Biotechnol 25, 275–281 (2016). https://doi.org/10.1007/s10068-016-0040-8

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  • DOI: https://doi.org/10.1007/s10068-016-0040-8

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