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Anti-hypertensive effect of grape seed extract in male spontaneously hypertensive rats

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Abstract

Grape seed extract contains high concentration of polyphenols that are well known as potent vasodilators. Herein, we investigated the blood pressure-lowering effect of enzyme-treated grape seed extract (ET-GSE) and the involvement of endothelium nitric oxide synthase (eNOS), which plays an important role in the regulation of vasodilation and relaxation of blood vessels in male spontaneously hypertensive rats (SHRs). Six-week-old male Wistar Kyoto rats and SHRs were orally administered ET-GSE (15 and 30 mg/kg/day) as treatments for 8 weeks. Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured by the tail-cuff method. After treatment for 8 weeks, thoracic aortas of sacrificed rats were harvested for detecting eNOS by Western blot analysis. SHRs treated with ET-GSE exhibited significantly lower SBP and DBP as well as higher expression of eNOS compared with control animals (p<0.05). These findings suggest that ET-GSE can contribute to regulate blood pressure through up-regulated eNOS expression for nitric oxide production.

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References

  1. Husserl FE, Messerli FH. Adverse effects of antihypertensive drugs. Drugs 22: 188–210 (1981)

    Article  CAS  Google Scholar 

  2. Joshi VD, Dahake AP, Suthar AP. Adverse effects associated with the use of antihypertensive drugs: An overview. Int. J. PharmTech Res. 2: 10–13 (2010)

    CAS  Google Scholar 

  3. Sivaprakasapillai B, Edirisinghe I, Randolph J, Steinberg F, Kappagoda T. Effect of grape seed extract on blood pressure in subjects with the metabolic syndrome. Metabolism 58: 1743–1746 (2009)

    Article  CAS  Google Scholar 

  4. Barona J, Aristizabal JC, Blesso CN, Volek JS, Fernandez ML. Grape polyphenols reduce blood pressure and increase flow-mediated vasodilation in men with metabolic syndrome. J. Nutr. 142: 1626–1632 (2012)

    Article  CAS  Google Scholar 

  5. Belcaro G, Ledda A, Hu S, Cesarone MR, Feragalli B, Dugall M. Grape seed procyanidins in pre-and mild hypertension: A registry study. Evid-Based Compl. Alt. Article ID 313142 (2013)

    Google Scholar 

  6. Ou K, Gu L. Absorption and metabolism of proanthocyanidins. J. Funct. Foods 7: 43–53 (2014)

    Article  CAS  Google Scholar 

  7. Thandapilly SJ, LeMaistre JL, Louis XL, Anderson CM, Netticadan T, Anderson HD. Vascular and cardiac effects of grape powder in the spontaneously hypertensive rat. Am. J. Hypertens. 25: 1070–1076 (2012)

    Article  CAS  Google Scholar 

  8. Quiñones M, Guerrero L, Suarez M, Pons Z, Aleixandre A, Arola L, Muguerza B. Low-molecular procyanidin rich grape seed extract exerts antihypertensive effect in males spontaneously hypertensive rats. Food Res. Int. 51: 587–595 (2013)

    Article  Google Scholar 

  9. Pons Z, Guerrero L, Margalef M, Arola L, Arola-Arnal A, Muguerza B. Effect of low molecular grape seed proanthocyanidins on blood pressure and lipid homeostasis in cafeteria diet-fed rats. J. Physiol. Biochem. 70: 629–637 (2014)

    Article  CAS  Google Scholar 

  10. Quiñones M, Guerrero L, Fernández-Vallinas S, Pons Z, Arola L, Aleixandre A, Muguerza B. Involvement of nitric oxide and prostacyclin in the antihypertensive effect of low-molecular-weight procyanidin rich grape seed extract in male spontaneously hypertensive rats. J. Funct. Foods 6: 419–427 (2014)

    Article  Google Scholar 

  11. FDA. Guidance for industry: Estimating the maximum safe starting dose in initial clinical trials for therapeutics in adult healthy volunteers. Food and Drug Administration, Rockville, MD, USA (2005)

  12. Scalbert A, Morand C, Manach C, Rémésy, C. Absorption and metabolism of polyphenols in the gut and impact on health. Biomed. Pharmacother. 56: 276–282 (2002)

    Article  CAS  Google Scholar 

  13. Déprez S, Mila I, Huneau J-F, Tomé D, Scalbert A. Transport of proanthocyanidin dimer, trimer, and polymer across monolayers of human intestinal epithelial Caco-2 cells. Antioxid. Redox Sign. 3: 957–967 (2001)

    Article  Google Scholar 

  14. Holt RR, Lazarus SA, Sullards MC, Zhu QY, Schramm DD, Hammerstone JF, Fraga CG, Schmitz HH, Keen CL. Procyanidin dimer B2 [epicatechin-(4²-8)-epicatechin] in human plasma after the consumption of a flavanol-rich cocoa. Am. J. Clin. Nutr. 76: 798–804 (2002)

    CAS  Google Scholar 

  15. Tsang C, Auger C, Mullen W, Bornet A, Rouanet JM, Crozier A, Teissedre PL. The absorption, metabolism and excretion of flavan-3-ols and procyanidins following the ingestion of a grape seed extract by rats. Brit. J. Nutr. 94: 170–181 (2005)

    Article  CAS  Google Scholar 

  16. Shrikhande AJ, Wang H, Kupina SA. Grape extract, dietary supplement thereof, and processes therefor. US Patent No. 8,075,929 B2 (2011)

    Google Scholar 

  17. Robinson M, Lu B, Edirisinghe I, Kappagoda CT. Effect of grape seed extract on blood pressure in subjects with pre-hypertension. J. Pharm. Nutr. Sci. 2: 155–159 (2012)

    CAS  Google Scholar 

  18. Edirisinghe I, Burton-Freeman B, Kappagoda CT. Mechanism of the endothelium-dependent relaxation evoked by a grape seed extract. Clin. Sci. 114: 331–337 (2008)

    Article  CAS  Google Scholar 

  19. Singleton VL, Rossi JA,. Colorimetry of total phenolics with phosphomolybdicphosphotungstic acid reagents. Am. J. Enol. Viticult. 16: 144–158 (1965)

    CAS  Google Scholar 

  20. Moyer RA, Hummer KE, Finn CE, Frei B, Wrolstad RE. Anthocyanins, phenolics, and antioxidant capacity in diverse small fruits: Vaccinium, Rubus, and Ribes. J. Agr. Food Chem. 50: 519–525 (2002)

    Article  CAS  Google Scholar 

  21. García-Falcón MS, Pérez-Lamela C, Martínez-Carballo E, Simal-Gándara J. Determination of phenolic compounds in wines: Influence of bottle storage of young red wines on their evolution. Food Chem. 105: 248–259 (2007)

    Article  Google Scholar 

  22. Šeruga M, Novak I, Jakobek L. Determination of polyphenols content and antioxidant activity of some red wines by differential pulse voltammetry, HPLC and spectrophotometric methods. Food Chem. 124: 1208–1216 (2011)

    Article  Google Scholar 

  23. Kim SH, Lee TH, Lee SM, Park JH, Park KH, Jung M, Jung H, Mohamed MAA, Baek NI, Chung IS, Kim JY. Cynandione A attenuates lipopolysaccharideinduced production of inflammatory mediators via MAPK inhibition and NF-κB inactivation in RAW 264.7 macrophages and protects mice against endotoxin shock. Exp. Biol. Med. 240: 946–954 (2015)

    Article  CAS  Google Scholar 

  24. Kruger MJ, Davies N, Myburgh KH, Lecour S. Proanthocyanidins, anthocyanins and cardiovascular diseases. Food Res. Int. 59:41–52 (2014)

    Article  CAS  Google Scholar 

  25. Mattivi F, Vrhovsek U, Masuero D, Trainotti D. Differences in the amount and structure of extractable skin and seed tannins amongst red grape varieties. Aust. J. Grape Wine R. 15: 27–35 (2009)

    Article  CAS  Google Scholar 

  26. Banaszewski K, Edirisinghe I, Kappagoda T, Burton-Freeman B, Zweigenbaum J, Cappozzo J. Detection and characterization of polyphenolic compounds and their metabolites in human plasma after consumption of grape seed extract (abstract no. 2221). In: Abstracts: 60th ASMS Conference. May 20-24, Vancouver Convention and Exhibition Centre, Vancouver, Canada. American Society for Mass Spectrometry, Santa Fe, NM, USA (2012)

    Google Scholar 

  27. Luo Y, Owens D, Mulder G, McVey A, Fisher T. Blood pressure characterization of hypertensive and control rats for cardiovascular studies. In: Abstracts: 62nd High Blood Pressure Research Conference. September 17-20, Omni Hotel at CNN Center, Atlanta, GA, USA. American Heart Association, Dallas, TX, USA (2008)

    Google Scholar 

  28. Cishek MB, Galloway MT, Karim M, German JB, Kappagoda CT. Effect of red wine on endothelium-dependent relaxation in rabbits. Clin. Sci. 93: 507–511 (1997)

    Article  CAS  Google Scholar 

  29. Bauer V, Sotníková R. Nitric oxide-The endothelium-derived relaxing factor and its role in endothelial functions. Gen. Physiol. Biophys. 29: 319–340 (2010)

    Article  CAS  Google Scholar 

  30. Lockette W, Otsuka Y, Carretero O. The loss of endothelium-dependent vascular relaxation in hypertension. Hypertension 8(suppl II): II-61-II-66 (1986)

    Google Scholar 

  31. Malinski T, Kapturczak M, Dayharsh J, Bohr D. Nitric oxide synthase activity in genetic hypertension. Biochem. Bioph. Res. Co. 194: 654–658 (1993)

    Article  CAS  Google Scholar 

  32. Stoclet J-C, Chataigneau T, Ndiaye M, Oak M-H, El Bedoui J, Chataigneau M, Schini-Kerth VB. Vascular protection by dietary polyphenols. Eur. J. Pharmacol. 500: 299–313 (2004)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Food Science and Biotechnology and Graduate School of Biotechnology, Kyung Hee University, Yongin, Gyeonggi, 17104, Korea

    Hyun-Ho Jang, Seung Kook Park & Dae-Ok Kim

  2. Graduate School of East-West Medical Sciences, Kyung Hee University, Yongin, Gyeonggi, 17104, Korea

    Ga-Young Choi & Ji-Ho Park

  3. Department of Genetic Engineering, Kyung Hee University, Yongin, Gyeonggi, 17104, Korea

    Tae-Hoon Lee & Ha-Na Jung

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  1. Hyun-Ho Jang
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Correspondence to Dae-Ok Kim.

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Jang, HH., Park, S.K., Choi, GY. et al. Anti-hypertensive effect of grape seed extract in male spontaneously hypertensive rats. Food Sci Biotechnol 24, 2229–2233 (2015). https://doi.org/10.1007/s10068-015-0297-3

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  • DOI: https://doi.org/10.1007/s10068-015-0297-3

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