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Cloning of the LEU2 gene from the amylolytic yeast Saccharomycopsis fibuligera

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Abstract

The SfLEU2 structural gene of LEU2 encoding β-isopropylmalate dehydrogenase was cloned from the amylolytic yeast Saccharomycopsis fibuligera isolated from Nuruk, a Korean traditional starter for rice wine production. Sequence analysis revealed the presence of an uninterrupted open-reading frame of 1,107 bp, including a stop codon, encoding a 368 amino acid residue. The deduced amino acid sequence of Leu2 in S. fibuligera was 76.4% homologous with Candida boidinii and Kluyveromyces marxianus sequences. The SfLEU2 gene complemented leu2Δ mutation in Saccharomyces cerevisiae, suggesting that the gene encodes a functional LEU2 in S. fibuligera. The GenBank Accession No. for SfLEU2 is KR820563.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Kangwon National University, Chuncheon, Gangwon, 24341, Korea

    Eun-Hee Park & Myoung-Dong Kim

  2. Fermented Food Science Division, Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Wanju-gun, 55365, Korea

    Soo-Hwan Yeo

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  1. Eun-Hee Park
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  2. Soo-Hwan Yeo
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  3. Myoung-Dong Kim
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Correspondence to Myoung-Dong Kim.

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Park, EH., Yeo, SH. & Kim, MD. Cloning of the LEU2 gene from the amylolytic yeast Saccharomycopsis fibuligera . Food Sci Biotechnol 24, 2151–2154 (2015). https://doi.org/10.1007/s10068-015-0286-6

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  • DOI: https://doi.org/10.1007/s10068-015-0286-6

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