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Purification, characterization, and DNA damage protection of active components from tartary buckwheat (Fagopyrum tataricum) hull

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Abstract

Isolation and purification of antioxidants with strongest activities using a guided strategy with 2,2,-diphenyl-1-picrylhydrazyl (DPPH) were performed. Crude extracts were obtained from Tartary buckwheat hulls using 70% ethanol. After polar-solvent extraction and purification using silica gel, Sephadex LH-20, and MCI gel CHP 20P column chromatography, the active component was subjected to structural identification. HPLC analysis indicated a high-purity yield for the purification process. Results of ultraviolet-visible spectrometry and HPLC with electrospray ionization mass spectrometry indicated that the main component was quercetin, with micro-quercetin-3-rhamnoside and kaempferol. Agarose gel electrophoresis revealed the potential of the purified active component as an in vitro protectant against DNA damage. Dose-dependent damage protection was observed for all tested concentrations. Quantitative analysis indicated the active component combines with radicals derived from H2O2 + UV treatment for termination of the radical chain reaction.

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Authors and Affiliations

  1. Department of Food Engineering, Sichuan University, Chengdu, 610065, China

    Xu Yang & Yuan-Ping Lv

  2. College of Agriculture and Life Sciences, Ankang University, Ankang, 725000, China

    Xu Yang

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Correspondence to Yuan-Ping Lv.

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Yang, X., Lv, YP. Purification, characterization, and DNA damage protection of active components from tartary buckwheat (Fagopyrum tataricum) hull. Food Sci Biotechnol 24, 1959–1966 (2015). https://doi.org/10.1007/s10068-015-0258-x

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  • DOI: https://doi.org/10.1007/s10068-015-0258-x

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