Abstract
The β-(1,3)-glucan originally isolated from Agrobacterium species was investigated for prompting maturation of dendritic cells (DCs) and driving Th1 immune responses. Bone-marrow derived DCs separated from mice were analyzed for augmentation of cell surface molecule (CD80, CD86, and major histo-compatability complex (MHC) class I/II) expression and pro-inflammatory cytokine production (tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6). β-Glucan functionally induced DCs activation via augmentation of CD80, CD86, and MHC class I/II expression and cytokine production (TNF-α, IL-1β, and IL-6). β-Glucan induced secretion of IL-12p70, but not IL-10. Both mitogen-activated protein kinases and nuclear factor (NF)-κB signaling mediated production of pro-inflammatory cytokine induced by β-glucan. β-Glucan-treated DCs exhibited accelerated proliferation of murine splenocytes with increased levels of interferon (IFN)-γ. β-Glucan regulates innate and adaptive immunity via DCs activation and Th1 polarization of immune responses.
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Byun, EB., Sung, NY., Park, SH. et al. β-(1,3)-glucan isolated from Agrobacterium species induces maturation of bone marrow-derived dendritic cells and drives Th1 immune responses. Food Sci Biotechnol 24, 1533–1540 (2015). https://doi.org/10.1007/s10068-015-0197-6
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DOI: https://doi.org/10.1007/s10068-015-0197-6