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Isolation, expression, and characterization of S-adenosyl-l-methionine synthetase from Lactobacillus paraplantarum 72815

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Abstract

The S-adenosyl-l-methionine (SAM) synthetase gene was mined from Lactobacillus paraplantarum 72815 isolated from the traditional Korean fermented food Kimchi. The gene was cloned and expressed in Escherichia coli to obtain recombinant SAM synthetase (SAM-s-LP). The purified SAM-s-LP was characterized as 40°C and pH 8.5 as the optimal temperature and pH for activity, respectively. The apparent K m values of SAM-s-LP were 1.47±0.59 mM for l-Met and 2.84±0.82 mM for ATP.

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References

  1. Lu SC. S-Adenosylmethionine. Int. J. Biochem. Cell B. 32: 391–395 (2000)

    Article  CAS  Google Scholar 

  2. Friedel HA, Goa KL, Benfield P. S-Adenosyl-L-methionine. A review of its pharmacological properties and therapeutic potential in liver dysfunction and affective disorders in relation to its physiological role in cell membranes. Drugs 38: 389–416 (1989)

    Article  CAS  Google Scholar 

  3. Lieber CS. Role of S-adenosyl-L-methionine in the treatment of liver diseases. J. Hepatol. 30: 1155–1159 (1999)

    Article  CAS  Google Scholar 

  4. Gatto G, Caleri D, Michelacci S, Sicuteri F. Analgesizing effect of a methyl donor (S-adenosylmethionine) in migraine: An open clinical trial. Int. J. Clin. Pharm. 6: 15–17 (1986)

    CAS  Google Scholar 

  5. Bottiglieri T. S-adenosyl-L-methionine (SAMe): From the bench to the beside-molecular basis of a pleiotrophic molecule. Am. J. Clin. Nutr. 76: 1151–1157 (2002)

    Google Scholar 

  6. Matos JR, Raushel FM, Wong CH. S-Adenosylmethionine: Studies on chemical and enzymatic synthesis. Biotechnol. Appl. Bioc. 9: 39–52 (1987)

    CAS  Google Scholar 

  7. Shobayashi M, Mukai N, Iwashita K, Hiraga Y, Iefuji H. A new method for isolation of S-adenosylmethionine (SAM)-accumulating yeast. Appl. Microbiol. Biot. 69: 704–710 (2006)

    Article  CAS  Google Scholar 

  8. Kim JY, Suh JW, Ji GE. Evaluation of S-adenosyl-L-methionine production by Bifidobacterium bifidum BGN4. Food Sci. Biotechnol. 17: 184–187 (2008)

    CAS  Google Scholar 

  9. Lee MK, Lee JK, Son JA, Kang MH, Koo KH, Suh JW. Sadenosyl-L-methionine (SAM) production by lactic acid bacteria strains isolated from different fermented kimchi products. Food Sci. Biotechnol. 17: 857–860 (2008)

    CAS  Google Scholar 

  10. Yoon GS, Ko KH, Kang HW, Suh JW, Kim YS, Ryu YW. Characterization of S-adenosylmethionine synthetase from Streptomyces avermitilis NRRL8165 and its effect on antibiotic production. Enzyme Microb. Tech. 39: 466–473 (2006)

    Article  CAS  Google Scholar 

  11. Luo Y, Yuan Z, Luo G, Zhao F. Expression of secreted His-tagged S-adenosylmethionine synthetase in the methylotrophic yeast Pichia pastoris and its characterization, one-step purification, and immobilization. Biotechnol. Progr. 24: 214–220 (2008)

    Article  CAS  Google Scholar 

  12. Zhu H, Shen YL, Wei DZ, Zhu JW. Cloning and characterization of the Serratia marcescens metK and pfs genes involved in AI-2-dependent quorum-sensing system. Mol. Cell Biochem. 315: 25–30 (2008)

    Article  CAS  Google Scholar 

  13. Hu H, Qian J, Chu J, Wang Y, Zhuang Y, Zhang S. DNA shuffling of methionine adenosyltransferase gene leads to improved S-adenosyl-L-methionine production in Pichia pastoris. J. Biotechnol. 141: 97–103 (2009)

    Article  CAS  Google Scholar 

  14. Hu H, Qian J, Chu J, Wang Y, Zhuang Y, Zhang S. Optimization of L-methionine feeding strategy for improving S-adenosyl-L-methionine production by methionine adenosyltransferase overexpressed Pichia pastoris. Appl. Microbiol. Biot. 83: 1105–1114 (2009)

    Article  CAS  Google Scholar 

  15. Naidu AS, Bidlack WR, Clemens RA. Probiotic spectra of lactic acid bacteria (LAB). Crit. Rev. Food Sci. 38: 13–126 (1999)

    Article  Google Scholar 

  16. Kim DJ, Huh JH, Yang YY, Kang CM, Lee IH, Hyun CG, Hong SK, Suh JW. Accumulation of S-adenosyl-L-methionine enhances production of actinorhodin but inhibits sporulation in Streptomyces lividans TK23. J. Bacteriol. 185: 592–600 (2003)

    Article  CAS  Google Scholar 

  17. Takusagawa F, Kamitori S, Markham GD. Structure and function of S-adenosylmethionine synthetase: Crystal structures of S-adenosylmethionine synthetase with ADP, BrADP, and PPi at 2.8 Å resolution. Biochemistry-US 35: 2586–2596 (1996)

    Article  CAS  Google Scholar 

  18. Schröder G, Eichel J, Breinig S, Schröder J. Three differentially expressed S-adenosylmethionine synthetases from Catharanthus roseus: Molecular and functional characterization. Plant Mol. Biol. 33: 211–222 (1997)

    Article  Google Scholar 

  19. McQueney MS, Markham GD. Investigation of monovalent cation activation of S-adenosylmethionine synthetase using mutagenesis and uranyl inhibition. J. Biol. Chem. 270: 18277–18284 (1995)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Division of Bioengineering, Incheon National University, Incheon, 406-772, Korea

    In-Tae Cha & Myung-Ji Seo

  2. Department of Life Science, Graduate School of Incheon National University, Incheon, 406-772, Korea

    Myung-Ji Seo

  3. Gut Microbiome Research Group, Korea Food Research Institute, Seongnam, Gyeonggi, 463-746, Korea

    Young-Do Nam

  4. Korea University of Science and Technology, Daejeon, 305-350, Korea

    Young-Do Nam

Authors
  1. In-Tae Cha
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  2. Young-Do Nam
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Correspondence to Myung-Ji Seo.

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Cha, IT., Nam, YD. & Seo, MJ. Isolation, expression, and characterization of S-adenosyl-l-methionine synthetase from Lactobacillus paraplantarum 72815. Food Sci Biotechnol 24, 1447–1451 (2015). https://doi.org/10.1007/s10068-015-0186-9

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  • DOI: https://doi.org/10.1007/s10068-015-0186-9

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