Abstract
The blue mussel (Mytilus edulis) is widely distributed in European waters but it is little consumed. Although several studies have been reported biological activities of mussels, the anti-adipogenic activity of blue mussel and the molecular mechanism in anti-obese activity remain unknown. Here, we prepared a water extract of blue mussel (BME) and its anti-adipogenic activity was assessed in 3T3-L1 cells. BME treatment dose-dependently inhibited 3T3-L1 lipid accumulation, as judged by Oil Red O staining and microscopic analysis. Consistent with this, BME treatment markedly suppressed mRNA expression levels of adipogenic genes, such as C/EBPβ, PPARγ, C/EBPα, and FAS, in 3T3-L1 mature adipocytes. BME-inhibited lipid accumulation in 3T3-L1 adipocytes was limited with treatment after adipocyte differentiation, particularly in the early and intermediate stages. We also showed that the Wnt/β-catenin signaling pathway is required in BME-inhibited 3T3-L1 adipogenesis. These finding may provide scientific evidence for the development of functional foods against obesity.
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Shon, MS., Kim, SK., Song, JH. et al. Anti-adipogenic activity of blue mussel (Mytilus edulis) extract by regulation of 3T3-L1 adipogenesis through Wnt/β-catenin signaling pathway. Food Sci Biotechnol 24, 315–321 (2015). https://doi.org/10.1007/s10068-015-0042-y
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DOI: https://doi.org/10.1007/s10068-015-0042-y