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Development of a genus-specific PCR combined with ARDRA for the identification of Leuconostoc species in kimchi

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Abstract

With Leuconostoc species being increasingly isolated from many fermented kimchi, a systematic identification of this genus is required, for which an amplified ribosomal DNA restriction analysis (ARDRA) has been developed. This method applies both polymerase chain reaction and restriction pattern analysis using a restriction enzyme. First, using specific primers that were designed from lactic acid bacteria (LAB) sequences, 217 bp specific to LAB was produced. Then, restriction analysis using the MspI was applied to identify these bacteria at the species level. This study demonstrates novel ARDRA technique for the identification of Leuconostoc species was successful in identifying according to the genomic species.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, 143-701, Korea

    Jung-Min Park & Jin-Man Kim

  2. Department of Food Science, Sun Moon University, Chungnam, 336-708, Korea

    Hoon Park

  3. Department of Food Technology and Services, Eulji University, Gyeonggi, 461-713, Korea

    Cheul-Young Yang

Authors
  1. Jung-Min Park
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  2. Cheul-Young Yang
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  3. Hoon Park
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  4. Jin-Man Kim
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Correspondence to Jin-Man Kim.

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Park, JM., Yang, CY., Park, H. et al. Development of a genus-specific PCR combined with ARDRA for the identification of Leuconostoc species in kimchi. Food Sci Biotechnol 23, 511–516 (2014). https://doi.org/10.1007/s10068-014-0070-z

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  • DOI: https://doi.org/10.1007/s10068-014-0070-z

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