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Effects of miR-150-5p on the growth and SOCS1 expression of rheumatoid arthritis synovial fibroblasts

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A Correction to this article was published on 16 January 2020

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Abstract

Objective

miR-150-5p has been implicated in the regulation and onset of immune diseases. We investigated the effects of miR-150-5p on the functions of RA synovial fibroblasts (RASFs).

Method

The binding site between suppressor of cytokine signaling 1 (SOCS1) and miR-150-5p was analyzed using European Bioinformatics Institute database, and the 3′ UTR of SOCS1 mRNA, including the binding site, was amplified and ligated to the 3′-end of LUC2 gene in the pmirGL0 dual-luciferase vector. The pmirGL0 vector and corresponding mimics were subsequently co-transfected into 293T cells to compare the relative fluorescence intensity of LUC2 between the miR-150-5p mimics and the negative control (NC) mimics groups. Further, the RASF cell line MH7A was transfected with miR-150-5p or NC mimics and subjected to flow cytometric analysis, cell counting kit-8 assay, western blot analysis, qPCR, and enzyme-linked immunosorbent (ELISA) assay 48 h after transfection.

Results

miR-150-5p mimics resulted in a lower cell apoptotic rate and proportion of cells in the S phase. Using a dual-luciferase reporter gene assay, we then found that SOCS1 is a potential target of miR-150-5p. Compared with NC mimics, miR-150-5p mimics significantly decreased the protein and mRNA expression levels of SOCS1. ELISA assay showed that miR-150-5p mimics increased interleukin-6 level in the cell culture medium but did not influence tumor necrosis factor-alpha levels.

Conclusions

Overall, the growth-promoting effect of miR-150-5p on MH7A cells may be attributed to the miR-150-5p-induced degradation of SOCS1 mRNA, suggesting a potential therapeutic target for RA.

Key Points

• SOCS1 is a potential target of miR-150-5p.

• miR-150-5p promoted the growth of RASF cell line MH7A.

• miR-150-5p increased the secretion of IL-6 but did not significantly affect TNF-α levels in MH7A cells.

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Data availability

The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.

Change history

  • 16 January 2020

    The first name of the co-author of the above article was presented incorrect in the published version. The author name “<Emphasis Type="Bold">Miangliang Qiu</Emphasis>” should read “<Emphasis Type="Bold">Mingliang Qiu</Emphasis>” as mentioned above.

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Funding

This study was partially supported by a grant from the project of National Natural Science Foundation of China (NO.21605075) and the project of Natural Science Foundation of Jiangxi Province (NO.20181BAB203020, NO.20192BBGL70024). The funder had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Authors and Affiliations

  1. Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China

    Mingliang Qiu & Juxiang Li

  2. Department of Rheumatology, The Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, 330006, China

    Mingliang Qiu, Lisha Mo & Weidong Xu

  3. Department of Clinical Laboratory, The Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, 330006, China

    Hua Liang

  4. Department of Pediatrics, The Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, 330006, China

    Weina Zhu

  5. Department of Chemistry, Nanchang University, Nanchang, 330031, China

    Xiangjuan Zheng

  6. Department of Rheumatology, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China

    Xinwang Duan

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  1. Mingliang Qiu
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Correspondence to Xinwang Duan or Weidong Xu.

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The original version of this article was revised: The first name of the co-author of the above article was presented incorrect in the published version. The author name “Miangliang Qiu” should read “Mingliang Qiu” and is now presented correctly in this article.

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Qiu, M., Mo, L., Li, J. et al. Effects of miR-150-5p on the growth and SOCS1 expression of rheumatoid arthritis synovial fibroblasts. Clin Rheumatol 39, 909–917 (2020). https://doi.org/10.1007/s10067-019-04894-7

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