Skip to main content
SpringerLink
Log in

Circulating endothelial progenitor cells in small-diameter artificial blood vessel

  • Review
  • Tissue Engineering / Regenerative Medicine
  • Published:
Journal of Artificial Organs Aims and scope Submit manuscript

Abstract

In the tissue engineering research field, the presence of circulating endothelial progenitor cells (EPCs) in the peripheral blood of adults represents a promising cell source to grow autologous endothelium on blood-contacting devices. Materials functionalized with EPC-specific molecules are an intriguing strategy to induce the homing and differentiation of the trapped EPCs into endothelial cells to generate a non-thrombogenic surface. Although the EPCs have been identified in adult peripheral blood about 30 years ago, in the subsequent literatures, the term “EPCs” has encompassed different cell populations with a mixed ability to contribute to the formation of blood vessels. This confusion is due to limited functional characterization of “EPCs”, an improper nomenclature, and the poorly standardized protocols. This review will screen the literature about “EPCs” to propose a specific nomenclature, phenotypic characterization, and, eventually, a protocol to perform reliable experiments

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Potente M, Gerhardt H, Carmeliet P. Basic and therapeutic aspects of angiogenesis. Cell. 2011;146:873–87.

    Article  CAS  Google Scholar 

  2. Gerhardt H, Golding M, Fruttiger M, Ruhrberg C, Lundkvist A, Abramsson A, et al. VEGF guides angiogenic sprouting utilizing endothelial tip cell filopodia. J Cell Biol. 2003;161:1163–77.

    Article  CAS  Google Scholar 

  3. Valaitis J, McGrew EA, McGrath RGRS. Capillary cell clusters inperipheral blood of postoperative cancerand noncancer patients. Acta Cytol. 1968;12:439.

    CAS  PubMed  Google Scholar 

  4. Stump MM, Jordan JL Jr, DeBakey MEHB. Endothelium grownfrom circulating blood on isolated intra-vascular Dacron hub. Am J Pathol. 1963;43:361–7.

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Mackenzie JR, Hackett M, Topuzlu CTD. Origin of arterial pros-thesis lining from circulating blood cells. Arch Surg. 1968;97:879–85.

    Article  CAS  Google Scholar 

  6. Asahara T, Murohara T, Sullivan A, Silver M, van der Zee R, Li T, et al. Isolation of putative progenitor endothelial cells for angiogenesis. Science. 1997;275:964–7.

    Article  CAS  Google Scholar 

  7. Testa U, Saulle E, Castelli G, Pelosi E. Endothelial progenitor cells in hematologic malignancies. Stem Cell Investig. 2016;3:26–26.

    Article  Google Scholar 

  8. Tomanek RJ, Schatteman GC. Angiogenesis: new insights and therapeutic potential. Anat Rec. 2000;261:126–35.

    Article  CAS  Google Scholar 

  9. Hur J, Yoon CH, Kim HS, Choi JH, Kang HJ, Hwang KK, et al. Characterization of two types of endothelial progenitor cells and their different contributions to neovasculogenesis. Arterioscler Thromb Vasc Biol. 2004;24:288–93.

    Article  CAS  Google Scholar 

  10. Hirschi KK, Ingram DA, Yoder MC. Assessing identity, phenotype, and fate of endothelial progenitor cells. Arterioscler Thromb Vasc Biol. 2008;28:1584–95.

    Article  CAS  Google Scholar 

  11. Medina RJ, O’Neill CL, Humphreys MW, Gardiner TA, Stitt AW. Outgrowth endothelial cells: characterization and their potential for reversing ischemic retinopathy. Investig Ophthalmol Vis Sci. 2010;51:5906–13.

    Article  Google Scholar 

  12. Siemerink MJ, Klaassen I, Vogels IMC, Griffioen AW, Van Noorden CJF, Schlingemann RO. CD34 marks angiogenic tip cells in human vascular endothelial cell cultures. Angiogenesis. 2012;15:151–63.

    Article  CAS  Google Scholar 

  13. Cheng C-C, Chang S-J, Chueh Y-N, Huang T-S, Huang P-H, Cheng S-M, et al. Distinct angiogenesis roles and surface markers of early and late endothelial progenitor cells revealed by functional group analyses. BMC Genom. 2013;14:182.

    Article  CAS  Google Scholar 

  14. Bou Khzam L, Bouchereau O, Boulahya R, Hachem A, Zaid Y, Abou-Saleh H, et al. Early outgrowth cells versus endothelial colony forming cells functions in platelet aggregation. J Transl Med BioMed Central. 2015;13:353.

    Article  Google Scholar 

  15. Joo HJ, Song S, Seo HR, Shin JH, Choi SC, Park JH, et al. Human endothelial colony forming cells from adult peripheral blood have enhanced sprouting angiogenic potential through up-regulating VEGFR2 signaling. Int J Cardiol. 2015;197:33–43. https://doi.org/10.1016/j.ijcard.2015.06.013.

    Article  PubMed  Google Scholar 

  16. Hebbel RP. Blood endothelial cells: utility from ambiguity. J Clin Investig. 2017;127:1613–5.

    Article  Google Scholar 

  17. Medina RJ, Barber CL, Sabatier F, Dignat-George F, Melero-Martin JM, Khosrotehrani K, et al. Endothelial progenitors: a consensus statement on nomenclature. Stem Cells Transl Med. 2017;6(5):1316–20.

    Article  Google Scholar 

  18. Shaik MV, Shaik M, Gangapatnam S. Analysis of endothelial progenitor subpopulation cells, oxidative DNA damage, and their role in coronary artery disease. Biomed Biotechnol Res J. 2018;2:136–41.

    Article  Google Scholar 

  19. Friedrich EB, Walenta K, Scharlau J, Nickenig G, Werner N, Cd CD, et al. CD34−/CD133+/VEGFR-2+ endothelial progenitor cell subpopulation with potent vasoregenerative capacities. Circ Res. 2006;98:20–5.

    Article  Google Scholar 

  20. Peichev M, Naiyer A, Pereira D, Zhu Z, Lane W, Williams M, et al. Expression of VEGFR-2 and AC133 bycirculating human CD34(+) cells identifies a population of functional endothelial precursors. Blood. 2000;95:952–8.

    Article  CAS  Google Scholar 

  21. Avci-Adali M, Perle N, Ziemer G, Wendel HP. Current concepts and new developments for autologous in vivo endothelialisation of biomaterials for intravascular applications. Eur Cell Mater. 2011;21:157–76.

    Article  CAS  Google Scholar 

  22. Formiga F, Pelacho B, Garbayo E, Abizanda G, Gavira J, Simon-Yarza T, et al. Sustained release of VEGF through PLGA microparticles improves vasculogenesis and tissue remodeling in an acute myocardial ischemia-reperfusion model. J Control Release. 2010;47:30–7.

    Article  Google Scholar 

  23. Hauser S, Jung F, Pietzsch J. Human endothelial cell models in biomaterial research. Trends Biotechnol. 2017;35:1–13.

    Article  Google Scholar 

  24. Salven P, Mustjoki S, Alitalo R, Alitalo K, Rafii S. VEGFR-3 and CD133 identify a population of CD34+ lymphatic/vascular endothelial precursor cells. Blood. 2003;101:168–72.

    Article  CAS  Google Scholar 

  25. Castelli G, Parolini I, Cerio AM, D’Angiò A, Pasquini L, Carollo M, et al. Conditioned medium from human umbilical vein endothelial cells markedly improves the proliferation and differentiation of circulating endothelial progenitors. Blood Cells Mol Dis. 2016;61:58–655.

    Article  CAS  Google Scholar 

  26. Parham KA, Pitson SM, Bonder CS. Regulation of EPCs: the gateway to blood vessel formation. New J Sci. 2014;2014:972043. https://doi.org/10.1155/2014/972043.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We gratefully acknowledge the financial support of the S-innovation Research Program for the “Development of the biofunctional materials for realization of innovative medicine”, Japan Agency for Medical Research and Development (AMED).

Author information

Authors and Affiliations

  1. Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Suita, 565-8565, Osaka, Japan

    Maria Chiara Munisso & Tetsuji Yamaoka

Authors
  1. Maria Chiara Munisso
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tetsuji Yamaoka
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

MCM: interpretation and manuscript writing. TY: interpretation and manuscript writing. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Maria Chiara Munisso.

Ethics declarations

Conflict of interest

The authors have no conflict of interest to declare.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Munisso, M.C., Yamaoka, T. Circulating endothelial progenitor cells in small-diameter artificial blood vessel. J Artif Organs 23, 6–13 (2020). https://doi.org/10.1007/s10047-019-01114-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10047-019-01114-6

Keywords

Search

Navigation