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Appropriate nonwoven filters effectively capture human peripheral blood cells and mesenchymal stem cells, which show enhanced production of growth factors

  • Original Article
  • Tissue Engineering / Regenerative Medicine
  • Published:
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Abstract

Scaffolds, growth factors, and cells are three essential components in regenerative medicine. Nonwoven filters, which capture cells, provide a scaffold that localizes and concentrates cells near injured tissues. Further, the cells captured on the filters are expected to serve as a local supply of growth factors. In this study, we investigated the growth factors produced by cells captured on nonwoven filters. Nonwoven filters made of polyethylene terephthalate (PET), biodegradable polylactic acid (PLA), or chitin (1.2–22 μm fiber diameter) were cut out as 13 mm disks and placed into cell-capturing devices. Human mesenchymal stem cells derived from adipose tissues (h-ASCs) and peripheral blood cells (h-PBCs) were captured on the filter and cultured to evaluate growth factor production. The cell-capture rates strongly depended on the fiber diameter and the number of filter disks. Nonwoven filter disks were composed of PET or PLA fibers with fiber diameters of 1.2–1.8 μm captured over 70 % of leukocytes or 90 % of h-ASCs added. The production of vascular endothelial growth factor (VEGF), transforming growth factor β1, and platelet-derived growth factor AB were significantly enhanced by the h-PBCs captured on PET or PLA filters. h-ASCs on PLA filters showed significantly enhanced production of VEGF. These enhancements varied with the combination of the nonwoven filter and cells. Because of the enhanced growth factor production, the proliferation of human fibroblasts increased in conditioned medium from h-PBCs on PET filters. This device consisting of nonwoven filters and cells should be investigated further for possible use in the regeneration of impaired tissues.

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Acknowledgments

The authors thank Mr. Tomohisa Inoue of Shinwa Co., Ltd., Mr. Yoshio Katsuta of Fusyokufu Joho, and Ms. Mikiko Ariga of Asahi Kasei Fibers Corporation for providing the nonwoven filters. The authors also thank Ms. Kasumi Suzuki, Ms. Takami Kobayashi, Ms. Miwa Sakata, Mr. Tomoyuki Miyazaki, Mr. Yuta Saito, and Mr. Shun Kobayashi for technical assistance. This work was partly supported by a research grant from Aichi Kidney Foundation and JSPS KAKENHI Grant Number 25410232.

Conflict of interest

Mikitomo Yasutake, General Manager, and Ushio Iwamoto, Chief Researcher, are employees of Asahi Kasei Corporation Co., Ltd. (Cell Therapy Technology, Healthcare R&D Center) and have the stock of the company. Yasuo Tokushima is an employee of Asahi Kasei Medical Co., Ltd. (Manager of Scientific and Technical Department, Japan Operation Division, Blood Purification Business Unit), and has the stock of Asahi Kasei Corporation Co., Ltd.. Nobuya Kitaguchi has the stock of Asahi Kasei Corporation Co., Ltd.. The other authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Faculty of Clinical Engineering, School of Health Sciences, Fujita Health University, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan

    Hideo Hori, Yoshiyuki Hiki, Kazunori Kawaguchi, Atsushi Ohashi, Shigeru Nakai & Nobuya Kitaguchi

  2. Healthcare R&D Center, Asahi Kasei Corporation, Tokyo, Japan

    Ushio Iwamoto & Mikitomo Yasutake

  3. Faculty of Joint Research Laboratory, Fujita Health University, Toyoake, Japan

    Gen Niimi

  4. Faculty of Medical Management and Information Science, School of Health Sciences, Fujita Health University, Toyoake, Japan

    Masanori Shinzato

  5. Therapeutic Apheresis Division, Asahi Kasei Medical, Co., Ltd, Tokyo, Japan

    Yasuo Tokushima

Authors
  1. Hideo Hori
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  2. Ushio Iwamoto
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  3. Gen Niimi
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  4. Masanori Shinzato
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  5. Yoshiyuki Hiki
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  6. Yasuo Tokushima
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  7. Kazunori Kawaguchi
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  8. Atsushi Ohashi
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  9. Shigeru Nakai
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  10. Mikitomo Yasutake
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  11. Nobuya Kitaguchi
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Corresponding author

Correspondence to Nobuya Kitaguchi.

Additional information

H. Hori, U. Iwamoto contributed equally.

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Hori, H., Iwamoto, U., Niimi, G. et al. Appropriate nonwoven filters effectively capture human peripheral blood cells and mesenchymal stem cells, which show enhanced production of growth factors. J Artif Organs 18, 55–63 (2015). https://doi.org/10.1007/s10047-014-0794-9

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  • DOI: https://doi.org/10.1007/s10047-014-0794-9

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