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Comparison of neovascularization in dermal substitutes seeded with autologous fibroblasts or impregnated with bFGF applied to diabetic foot ulcers using laser Doppler imaging

  • Original Article
  • Artificial Skin, Muscle, Bone / Joint, Neuron
  • Published:
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Abstract

A bilayered artificial dermis (AD) composed of an upper silicone sheet and a lower collagen sponge has been widely applied for skin defects. After application, fibroblasts and capillaries infiltrate the AD and the collagen sponge is replaced by host dermal tissue within a few weeks. However, this delay and the high incidence of infection are concerns regarding the use of AD in the treatment of chronic ulcers. In this study, we compared the neovascularization of conventional AD seeded with autologous fibroblasts (cultured dermis: CD) and collagen/gelatin sponge (CGS), which is a novel artificial dermis capable of sustained release of basic fibroblast growth factor (bFGF) after application using laser Doppler imaging (LDI). CD (n = 5) and CGS impregnated with bFGF (n = 6) were applied to diabetic foot ulcers after debridement. Perfusion units (PUs) were measured just after, and 1, 2 and 3 weeks after application, and complete healing rates within 16 weeks were compared. No significant differences in PUs were seen 1, 2 and 3 weeks after application and in healing rates within 16 weeks between the two groups. This study suggested that CD and CGS treatments were effective, but there were no significant differences between them in the treatment of diabetic ulcers .

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Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Plastic and Reconstructive Surgery, Kansai Medical University, 2-5-1 Shin-machi, Hirakata, 573-1010, Japan

    Naoki Morimoto, Natsuko Kakudo, Priscilla Valentin Notodihardjo & Kenji Kusumoto

  2. Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan

    Shigehiko Suzuki

Authors
  1. Naoki Morimoto
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  2. Natsuko Kakudo
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  3. Priscilla Valentin Notodihardjo
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  4. Shigehiko Suzuki
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  5. Kenji Kusumoto
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Corresponding authors

Correspondence to Naoki Morimoto or Natsuko Kakudo.

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Morimoto, N., Kakudo, N., Valentin Notodihardjo, P. et al. Comparison of neovascularization in dermal substitutes seeded with autologous fibroblasts or impregnated with bFGF applied to diabetic foot ulcers using laser Doppler imaging. J Artif Organs 17, 352–357 (2014). https://doi.org/10.1007/s10047-014-0782-0

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  • DOI: https://doi.org/10.1007/s10047-014-0782-0

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