Abstract
Four enzymes involved in sucrose metabolism: sucrose phosphate synthase (Sps), sucrose phosphate phosphatase (Spp), sucrose synthase (Sus) and fructokinase (FruK), were obtained as his-tagged proteins from the moderately thermophilic methanotroph Methylocaldum szegediense O12. Sps, Spp, FruK and Sus demonstrated biochemical properties similar to those of other bacterial counterparts, but the translated amino acid sequences of Sps and Spp displayed high divergence from the respective microbial enzymes. The Sus of M. szegediense O12 catalyzed the reversible reaction of sucrose cleavage in the presence of ADP or UDP and preferred ADP as a substrate, thus implying a connection between sucrose and glycogen metabolism. Sus-like genes were found only in a few methanotrophs, whereas amylosucrase was generally used in sucrose cleavage in this group of bacteria. Like other microbial fructokinases, FruK of M. szegediense O12 showed a high specificity to fructose.
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The work was supported by the Russian Foundation for Basic Research #16-04-00462-a and by the Russian Scientific Foundation #18-14-00326. The authors are grateful to all members of the Organization for Methanotroph Genome Analysis for their collaboration (OMeGA), the U.S. Department of Energy Joint Genome Institute and Genoscope for the access to methanotrophic genomes for comparative analyses.
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But, S.Y., Solntseva, N.P., Egorova, S.V. et al. The genes and enzymes of sucrose metabolism in moderately thermophilic methanotroph Methylocaldum szegediense O12. Extremophiles 22, 433–445 (2018). https://doi.org/10.1007/s00792-018-1006-y
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DOI: https://doi.org/10.1007/s00792-018-1006-y