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Bacterial distribution and inflammatory cytokines associated with oral cancer with and without jawbone invasion—a pilot study

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Abstract

Objective

To explore the bacterial and inflammatory variations in oral cancer patients with and without jawbone invasion.

Materials and methods

A total of 20 specimens of fresh tumor tissue, including 10 from the tumor-invaded jawbone (JIOC group) and 10 without jawbone invasion (NJIOC group), were collected from oral cancer patients. Meanwhile, 10 specimens from normal oral mucosa were collected from healthy patients (control group). The microbiomic content of each sample was analyzed by 16S rRNA gene sequencing, while the expression of inflammatory cytokines was assessed using protein microarray analysis.

Results

There was a significant difference in β diversity between JIOC and NJIOC groups (P < 0.05), but no difference between NJIOC and control groups. The average relative abundance of Fusobacteria and Spirochaetes was higher, while Firmicutes was lower in the JIOC group than in the NJIOC group (all P < 0.05). The expression of pro-inflammatory cytokines like interleukin (IL)-1α, IL-1β, IL-4, and IL-8 was upregulated in the JIOC group compared with the NJIOC group, while MCP-1 was decreased (all P < 0.05). Slackia spp. and Howardella spp. were positively correlated with IL-4; Odoribacter spp. and Acidaminococcaceae spp. were negatively correlated with IL-4, and Clostridium XIVa spp. was negatively correlated with IL-1α and IL-1β.

Conclusion

Bacterial and inflammatory differences were observed in oral cancer patients with and without jawbone invasion, where the relative abundance of the differential bacteria was associated with the expression of the inflammatory cytokines.

Clinical relevance

This study investigated the changes in the flora during jawbone invasion in oral cancer and its effect on inflammatory factors, elucidating the possible mechanisms of jawbone invasion caused by oral cancer, which may lead to new ideas for the clinical prevention and treatment of jawbone invasion in oral cancer.

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Acknowledgements

The authors also acknowledge the support of Peking University School and the Hospital of Stomatology for data acquisition for Table 1.

Funding

This study was funded by the National Natural Science Foundation of China (81900979 and 81972540), Beijing Municipal Natural Science Foundation (7212137), and the National Clinical Key Discipline Construction Project (PKUSSNKP-202114).

Author information

Author notes

  1. Yuanning Yang and Qingxiang Li contributed equally to this work.

Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, NO, 22, Zhongguancun South Street, Haidian District, Beijing, 100081, People’s Republic of China

    Yuanning Yang, Qingxiang Li, Qiao Qiao, Ning Zhao, Hongyuan Huang, Ying Zhou, Chuanbin Guo & Yuxing Guo

  2. National Clinical Research Center for Oral Diseases, Beijing, 100081, People’s Republic of China

    Yuanning Yang, Qingxiang Li, Qiao Qiao, Ning Zhao, Hongyuan Huang, Ying Zhou, Chuanbin Guo & Yuxing Guo

  3. National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing, 100081, People’s Republic of China

    Yuanning Yang, Qingxiang Li, Qiao Qiao, Ning Zhao, Hongyuan Huang, Ying Zhou, Chuanbin Guo & Yuxing Guo

  4. Beijing Key Laboratory of Digital Stomatology, Peking University School and Hospital of Stomatology, Beijing, 100081, People’s Republic of China

    Yuanning Yang, Qingxiang Li, Qiao Qiao, Ning Zhao, Hongyuan Huang, Ying Zhou, Chuanbin Guo & Yuxing Guo

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Contributions

Yuxing Guo, Yuanning Yang, and Qingxiang Li contributed to the conception, design, and data acquisition; drafted the manuscript; and critically revised the manuscript. Qiao Qiao, Ning Zhao, Hongyuan Huang, and Ying Zhou contributed to the design, data acquisition, drafting the manuscript, and critical revision of the manuscript. Chuanbin Guo contributed to the design, interpretation, drafting of the manuscript, and critical revision of the manuscript. All authors gave final approval and agreed to be accountable for all aspects of the work.

Corresponding author

Correspondence to Yuxing Guo.

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Ethics approval

Approval was obtained from the ethics committee of the Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology (PKUSHS). The procedures used in this study adhere to the tenets of the Declaration of Helsinki.

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Informed consent was obtained from all individual participants included in the study.

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The participants have consented to the submission of the case report to the journal and signed informed consent regarding publishing their data.

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The authors declare no competing interests.

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Yang, Y., Li, Q., Qiao, Q. et al. Bacterial distribution and inflammatory cytokines associated with oral cancer with and without jawbone invasion—a pilot study. Clin Oral Invest 27, 7285–7293 (2023). https://doi.org/10.1007/s00784-023-05319-1

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  • DOI: https://doi.org/10.1007/s00784-023-05319-1

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