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Chemical analysis of irradiated root dentin and its interaction with resin cements

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Abstract

Objectives

To investigate the chemical changes in root dentin submitted to ionizing radiation and how it affects the interaction with resin cements.

Materials and methods

Forty human premolars were randomly divided into two groups (n = 20): non-irradiated and irradiated. They were randomly subdivided according to the type of resin cement (n = 10): conventional (RelyX ARC, 3 M ESPE) or self-adhesive (RelyX U200, 3 M ESPE). After cementation of the fiberglass posts, the roots were sectioned to be analyzed by Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, and confocal laser scanning microscopy (CLSM). The data obtained from FTIR and Raman were analyzed using two-way ANOVA followed Tukey’s test (α = 0.05). For CLSM, a descriptive analysis was performed.

Results

In the FTIR, there was a significant difference between the non-irradiated and irradiated groups for phosphate (p = 0.011), carbonate (p < 0.001), amide III (p = 0.038), and carbonate/mineral ratio (p < 0.001). Regarding the root third, there was a difference for amide I (p = 0.002), mineral/matrix ratio (p = 0.001), and amide I/CH2 (p = 0.026) between the cervical and the others. Raman spectroscopy revealed no difference between groups for 961/1458 cm−1 in the diffusion zone. CLSM showed a different interaction pattern for the two cements with the irradiated dentin from the cervical third.

Conclusions

Ionizing radiation altered the chemical composition of root dentin, especially in the cervical third. The resin cements showed less interaction with the irradiated root dentin.

Clinical relevance

As radiotherapy alters the chemical composition of root dentin, the interaction of resin cement with dentin can compromise the success of rehabilitation with fiberglass posts.

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Funding

The study was supported by FAPEMIG, CNPq, and CAPES (Brazil) for the granting of scholarship and resource for the purchase of consumer material.

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Authors and Affiliations

  1. Department of Operative Dentistry and Dental Materials, School of Dentistry, Federal University of Uberlândia, Av Pará 1720, Block 4L, Campus Umuarama, Uberlândia, MG, 38405-320, Brazil

    Alessandro Marcelo Pelloso, Rafael Resende de Miranda, Matheus Elias Rossi & Veridiana Resende Novais

  2. Radiotherapy Sector, Clinical Hospital, Federal University of Triângulo Mineiro, Av Getúlio Guaritá 130, Uberaba, MG, 38025-440, Brazil

    Adriano Luiz Balthazar Bianchini & Francisco Américo Silveira Marcelino

  3. Postgraduate Program in Dentistry, School of Dentistry, CEUMA University, Rua Josué Montello 01, São Luís, MA, 65075-120, Brazil

    Edna Lúcia Coutinho da Silva

Authors
  1. Alessandro Marcelo Pelloso
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  2. Rafael Resende de Miranda
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  3. Matheus Elias Rossi
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  4. Adriano Luiz Balthazar Bianchini
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  5. Francisco Américo Silveira Marcelino
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  7. Veridiana Resende Novais
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Correspondence to Veridiana Resende Novais.

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Pelloso, A.M., de Miranda, R.R., Rossi, M.E. et al. Chemical analysis of irradiated root dentin and its interaction with resin cements. Clin Oral Invest 26, 4315–4325 (2022). https://doi.org/10.1007/s00784-022-04395-z

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