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3D quantification of in vivo orthodontic tooth movement in rats by means of micro-computed tomography

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A Correction to this article was published on 05 March 2022

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Abstract

Objective

(1) To test the accuracy of split-mouth models in rats for the study of orthodontic tooth movement (OTM) and (2) to propose an improved 3D model for quantification of OTM in rats.

Methods

Eleven Wistar rats were split into group 1 (dental anchorage) and group 2 (skeletal anchorage). In both groups, no orthodontic force (OF) was applied on the contralateral hemi-maxilla. In vivo micro-CT images were taken before (T0) and 31 days (T1) after OF. OTM was compared between time-points and experimental sides using conventional 2D analysis and a novel 3D model.

Results

Using incisors as anchorage leads to their distal displacement in both OF and no OF sides. In the OF side, movement of M1 is underestimated by incisor displacement. Mesial displacement of M1 was found in the no OF side of all groups 31 days after the application of OF.

Conclusions

The new 3D model yielded higher sensitivity for tooth displacement in planes other than sagittal and incisor displacement was reduced by using skeletal anchorage.

Clinical significance

Studies following split-mouth designs in orthodontic research in rats might be systematically underestimating the effects of techniques and/or medication on OTM, since there is tooth displacement on the control side. 3D quantification of OTM with skeletal anchorage is more sensitive and avoids displacement of the dental units used as anchorage.

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Acknowledgements

The authors thank Prof. Greetje Vande Velde, Mr. Jordi Penedo-Wijdmeier, Ms. Stephanie de Vleeschauwer, and Ms. Marie Roelandt (KU Leuven, Belgium) for their help with the animal experiments.

Funding

Author Chen Zong is supported by the China Scholarship Council (File No. 201806270252). Payments are made directly to him to support his doctoral studies (4-year allowance 2019–2023). This only covers his personal life expenses; no other funding has been received for the elaboration of this research.

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Author notes

  1. The authors Maria Cadenas de Llano-Pérula and Chen Zong contributed equally to this work.

Authors and Affiliations

  1. Department of Oral Health Sciences – Orthodontics, KU Leuven and University Hospitals Leuven, Kapucijnenvoer 7, blok A, bus 7001, 3000, Leuven, Belgium

    Maria Cadenas de Llano-Pérula, Chen Zong & Guy Willems

  2. Oral and Maxillofacial Surgery, University Hospitals Leuven and OMFS-IMPATH Research Group, Department of Imaging & Pathology, Faculty of Medicine, KU Leuven, Leuven, Belgium

    Jeroen Van Dessel

  3. Department of Orthodontics and Dentofacial Orthopedics, School of Dental Medicine/Medical Faculty, University of Bern, Bern, Switzerland

    Anne Marie Kuijpers-Jagtman

  4. Department of Orthodontics, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands

    Anne Marie Kuijpers-Jagtman

  5. Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

    Anne Marie Kuijpers-Jagtman

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  1. Maria Cadenas de Llano-Pérula
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  2. Chen Zong
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  3. Jeroen Van Dessel
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Corresponding author

Correspondence to Maria Cadenas de Llano-Pérula.

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Cadenas de Llano-Pérula, M., Zong, C., Van Dessel, J. et al. 3D quantification of in vivo orthodontic tooth movement in rats by means of micro-computed tomography. Clin Oral Invest 26, 3911–3920 (2022). https://doi.org/10.1007/s00784-021-04358-w

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