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Effect of association of non-steroidal anti-inflammatory and antibiotic agents with calcium hydroxide pastes on their cytotoxicity and biocompatibility

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Abstract

Objectives

Evaluate the cytotoxicity and biocompatibility of calcium hydroxide paste [Ca (OH)2] associated with 5% diclofenac sodium, ibuprofen, or amoxicillin.

Materials and methods

Pre-osteoblast-like cells were cultivated and the MTT test was used to determine the cytotoxicity of the paste extracts after time intervals of 24, 48, 72 h, and 7 days. Tubes containing Ca (OH)2 pastes associated with the drugs and empty tubes were implanted in subcutaneous tissue of 30 rats. After 7 and 30 days, the specimens were removed and submitted to histological analysis. The data obtained were submitted to Kruskal-Wallis and Dunn tests (p ≤ 0.05).

Results

All the Ca (OH)2 pastes promoted cell viability after all periods. At 7 days, there was greater inflammatory tissue reaction adjacent to the implants. At 30 days, there was a significant reduction in the number of inflammatory cells, and increase in fibroblasts in all groups. In this period, a lower number of inflammatory cells and a higher number of fibroblasts were observed in the capsules adjacent to the association with diclofenac, when compared with the other mixtures (p ≤ 0.05); the capsule thickness was greater at 7 days than at 30 days.

Conclusions

The Ca (OH)2 pastes associated with the drugs were not cytotoxic and presented biocompatibility after implantation in rat subcutaneous tissues.

Clinical relevance

Ca (OH)2 pastes with anti-inflammatory or antibiotic may be clinical alternatives as intracanal medication to reduce resistant microorganisms in root canal system.

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Authors and Affiliations

  1. Pro-Rectory of Research and Post-Graduation, Universidade do Sagrado Coração, Rua Irmã Arminda, 10-50, Bauru, SP, Brazil

    Guilherme Ferreira da Silva, Alan Matheus Ramos Garcia & Paulo Henrique Weckwerth

  2. Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil

    Francine Cesário, Marco Antônio Húngaro Duarte & Rodrigo Ricci Vivan

  3. Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil

    Rodrigo Cardoso de Oliveira

Authors
  1. Guilherme Ferreira da Silva
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  2. Francine Cesário
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  3. Alan Matheus Ramos Garcia
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  4. Paulo Henrique Weckwerth
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  5. Marco Antônio Húngaro Duarte
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  6. Rodrigo Cardoso de Oliveira
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  7. Rodrigo Ricci Vivan
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Correspondence to Guilherme Ferreira da Silva.

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da Silva, G.F., Cesário, F., Garcia, A.M.R. et al. Effect of association of non-steroidal anti-inflammatory and antibiotic agents with calcium hydroxide pastes on their cytotoxicity and biocompatibility. Clin Oral Invest 24, 757–763 (2020). https://doi.org/10.1007/s00784-019-02923-y

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  • DOI: https://doi.org/10.1007/s00784-019-02923-y

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