Abstract
Objective
To evaluate the effect of a fluoride toothpaste containing nano-sized sodium hexametaphosphate (HMPnano) on enamel demineralization on the biochemical composition and insoluble extracellular polysaccharide (EPS) in biofilm formed in situ.
Methods
This crossover double-blind study consisted of four phases (7 days each), in which 12 volunteers wore intraoral appliances containing four enamel bovine blocks. The cariogenic challenge was performed using 30% sucrose solution (6×/day). Blocks were treated 3×/day with the following toothpastes: no F/HMP/HMPnano (Placebo), conventional fluoride toothpaste, 1100 ppm F (1100F), 1100F + 0.5% micrometric HMP (1100F/HMP), and 1100F + 0.5% nano-sized HMP (1100F/HMPnano). The percentage of surface hardness loss (%SH), integrated loss of subsurface hardness (ΔKHN), and enamel calcium (Ca), phosphorus (P), and fluoride (F) were determined. Moreover, biofilms formed on the blocks were analyzed for F, Ca, P, and insoluble extracellular polysaccharide (EPS) concentrations. Data were analyzed using one-way ANOVA, followed by Student–Newman–Keuls’ test (p < 0.001).
Results
1100F/HMPnano promoted the lowest %SH and ΔKHN among all groups (p < 0.001). The addition of HMPnano to 1100F significantly increased Ca concentrations (p < 0.001). The 1100F/HMPnano promoted lower values of EPS when compared with 1100F (~ 70%) (p < 0.001) and higher values of fluoride and calcium in the biofilms (p < 0.001).
Conclusion
1100F/HMPnano demonstrated a greater protective effect against enamel demineralization and on the composition of biofilm in situ when compared to 1100F toothpaste.
Clinical relevance
This toothpaste could be a viable alternative to patients at high risk of caries.
Similar content being viewed by others
References
Bratthal D, Hansel-Petersson G, Sundberg H (1996) Reasons for the caries decline: what do the experts believe? Eur J Oral Sci 104:416–422
Danelon M, Pessan JP, Neto FN, de Camargo ER, Delbem AC (2015) Effect of toothpaste with nano-sized trimetaphosphate on dental caries: in situ study. J Dent 43:806–813. https://doi.org/10.1016/j.jdent.2015.04.010
da Camara DM, Pessan JP, Francati TM, Souza JA, Danelon M, Delbem AC (2016) Fluoride toothpaste supplemented with sodium hexametaphosphate reduces enamel demineralization in vitro. Clin Oral Investig 20:1981–1985. https://doi.org/10.1007/s00784-015-1707-x
Dalpasquale G, Delbem ACB, Pessan JP, Nunes GP, Gorup LF, Souza-Neto FN, Camargo ER, Danelon M (2017) Effect of the addition of nano-sized sodium hexametaphosphate to fluoride toothpastes on tooth demineralization: an in vitro study. Clin Oral Investig 21:1821–1827. https://doi.org/10.1007/s00784-017-2093-3
da Camara DM, Pessan JP, Francati TM, Santos Souza JA, Danelon M, Delbem AC (2015) Synergistic effect of fluoride and sodium hexametaphosphate in toothpaste on enamel demineralization in situ. J Dent 43:1249–1254. https://doi.org/10.1016/j.jdent.2015.08.007
Vaara M, Jaakkola J (1989) Sodium hexametaphosphate sensitizes Pseudomonas aeruginosa, several other species of Pseudomonas, and E. coli to hydrophobic drugs. Antimicrob Agents Chemother 3:1741–1747
Shibata H, Morioka T (1982) Antibacterial action of condensed phosphates on the bacterium Streptococcus mutans and experimental caries in the hamster. Arch Oral Biol 27:809–816. https://doi.org/10.1016/0003-9969(82)90034-6
Samiei M, Farjami A, Dizaj SM, Lotfipour F (2016) Nanoparticles for antimicrobial purposes in Endodontics: a systematic review of in vitro studies. Mater Sci Eng C 58:1269–1278. https://doi.org/10.1016/j.msec.2015.08.070
Zhang L, Pornpattananangku D, Hu CM, Huang CM (2010) Development of nanoparticles for antimicrobial drug delivery. Curr Med Chem 17:585–594. https://doi.org/10.2174/092986710790416290
He L, Deng D, Zhou X, Cheng L, ten Cate JM, Li J, Li X, Crielaard W (2015) Novel tea polyphenol-modified calcium phosphate nanoparticle and its remineralization potential. J Biomed Mater Res B Appl Biomater 8:1525–1531. https://doi.org/10.1002/jbm.b.33333
do Amaral JG, Martinhon CC, Delbem ACB (2013) Effect of low-fluoride toothpastes supplemented with calcium glycerophosphate on enamel demineralization in situ. Am J Dent 26:75–80
Delbem AC, Carvalho LP, Morihisa RK, Cury JÁ (2005) Effect of rinsing with water immediately after APF gel application on enamel demineralization in situ. Caries Res 39:258–260. https://doi.org/10.1159/000084808
Delbem AC, Sassaki KT, Vieira AE, Rodrigues E, Bergamaschi M, Stock SR, Cannon ML, Xiao X, De Carlo F, Delbem ACB (2009) Comparison of methods for evaluating mineral loss: hardness versus synchrotron microcomputed tomography. Caries Res 43:359–365. https://doi.org/10.1159/000231573
Danelon M, Takeshita EM, Sassaki KT, Delbem ACB (2013) In situ evaluation of a low fluoride concentration gel with sodium trimetaphosphate in enamel remineralization. Am J Dent 26:15–20
Spiguel MH, Tovo MF, Kramer PF, Franco KS, Alves KM, Delbem AC (2009) Evaluation of laser fluorescence in the monitoring of the initial stage of the de-/remineralization process: an in vitro and in situ study. Caries Res 43:302–307. https://doi.org/10.1159/000218094
Weatherell JA, Robinson C, Strong M, Nakagaki H (1985) Micro-sampling by abrasion. Caries Res 19:97–102. https://doi.org/10.1159/000260835
Alves KM, Pessan JP, Brighenti FL, Franco KS, Oliveira FA, Buzalaf MA, Sassaki KT, Delbem AC (2007) In vitro evaluation of the effectiveness of acidic fluoride dentifrices. Caries Res 41:263–267. https://doi.org/10.1159/000101915
Vogel GL, Chow LC, Brow WL (1983) A microanalytical procedure for the determination of calcium, phosphate and fluoride in enamel biopsy samples. Caries Res 17:23–31
Fiske CH, Subbarow Y (1925) The colorimetric determination of phosphorus. J Biol Chem 66:375–400
Nobre dos Santos M, Melo dos Santos L, Francisco SB, Cury JÁ (2002) Relationship among dental plaque composition, daily sugar exposure and caries in the primary dentition. Caries Res 36:347–352. https://doi.org/10.1159/000065959
Ccahuana-Vasquez RA, Tabchoury CPM, Tenuta LMA, Del Bel Cury AA, Vale GC, Cury JA (2007) Effect of frequency of sucrose exposure on dental biofilm composition and enamel demineralization in the presence of fluoride. Caries Res 41:9–15. https://doi.org/10.1159/000096100
Dubois M, Grilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356. https://doi.org/10.1021/ac60111a017
Danelon M, Pessan JP, Souza-Neto FN, de Camargo ER, Delbem AC (2017) Effect of fluoride toothpaste with nano-sized trimetaphosphate on enamel demineralization: an in vitro study. Arch Oral Biol 78:82–87. https://doi.org/10.1016/j.archoralbio.2017.02.014
van Dijk JW, Borggreven JM, Driessens FC (1980) The effect of some phosphates and a phosphonate on the electrochemical properties of bovine enamel. Arch Oral Biol 25:591–595. https://doi.org/10.1016/0003-9969(80)90072-2
Whitford GM, Wasdin JL, Schafer TE, Adair SM (2002) Plaque fluoride concentrations are dependent on plaque calcium concentrations. Caries Res 36:256–265. https://doi.org/10.1159/000063931
Pessan JP, Sicca CM, de Souza TS, da Silva SMB, Whitford GM, Buzalaf MAR (2006) Fluoride concentrations in dental plaque and saliva after the use of a fluoride toothpaste preceded by a calcium lactate rinse. Eur J Oral Sci 114:489–493. https://doi.org/10.1111/j.1600-0722.2006.00409.x
Pessan JP, Silva SMB, Lauris JRP, Sampaio FC, Whitford GM, Buzalaf MAR (2008) Fluoride uptake by plaque from water and from toothpaste. J Dent Res 87:461–465. https://doi.org/10.1177/154405910808700501
Cochrane HJ, Saranathan S, Cai F, Cross KJ, Reynolds EC (2008) Enamel subsurface lesion remineralisation with casein phosphopeptide stabilized solutions of calcium phosphate and fluoride. Caries Res 42:88–97. https://doi.org/10.1159/000113161
Boyd RF (1978) The effect of some divalent cations on extracellular polysaccharide synthesis in Streptococcus salivarius. J Dent Res 57:380–383
Van Loveren B (2001) Antimicrobial activity of fluoride and its in vivo importance: identification of research questions. Caries Res 35:65–70. https://doi.org/10.1159/000049114
Marquis RE, Clock SA, Mota-Meira M (2003) Fluoride and organic weak acids as modulators of microbial physiology. FEMS Microbiol Rev 26:493–510. https://doi.org/10.1111/j.1574-6976.2003.tb00627.x
Choi IK, Wen WW, Smith RW (1993) Technical note the effect of a long chain phosphate on the adsorption of collectors on kaolinite. Miner Eng 6:1191–1197
van Wazer JR, Campanella DA (1950) Structure and properties of the condensed phosphates. IV. Complex ion formation in polyphosphate solutions. J Am Chem Soc 72:655–663. https://doi.org/10.1021/ja01158a004
Acknowledgments
We thank the volunteers for their participation in the study, CAPES (Coordination of Higher Education Personnel), FAPESP (The State of São Paulo Research Foundation, grant 2016/03148-7), and CNPq/PQ (National Council for Scientific and Technological Development, grant 308981/2014-6) for the concession of a scholarship to the first, second, fourth, and eighth authors, respectively.
Funding
This study was supported by CAPES (Coordination of Higher Education Personnel), FAPESP (The State of São Paulo Research Foundation, grant 2016/03148-7), and CNPq/PQ (National Council for Scientific and Technological Development, grant 308981/2014-6).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors Marcelle Danelon, Alberto Carlos Botazzo Delbem, Juliano Pelim Pessan, and Emerson Rodrigues de Camargo hold a patent request for a product used in the study, by the National Institute of Industrial Property—INPI/SP, on October 17, 2014 under number BR 10 2014 025902 3.
Ethical approval
This study was approved by the Human Ethical Committee of São Paulo State University (UNESP), School of Dentistry, Araçatuba, Brazil (Protocol: 58549716.8.0000.5420).
Informed consent
Informed consent was obtained from all individual participants included in the study.
Rights and permissions
About this article
Cite this article
Garcia, L.S.G., Delbem, A.C.B., Pessan, J.P. et al. Anticaries effect of toothpaste with nano-sized sodium hexametaphosphate. Clin Oral Invest 23, 3535–3542 (2019). https://doi.org/10.1007/s00784-018-2773-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00784-018-2773-7