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Herpesviral-bacterial co-infection in mandibular third molar pericoronitis

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Abstract

Objective

The aim of this study was to assess the presence of herpesviruses and periodontopathic bacteria and to establish their potential association with pericoronitis.

Materials and methods

Fifty samples obtained with paper points (30 from pericoronitis and 20 controls) were subjected to polymerase chain reaction (PCR) analysis. A single-stage and nested PCR assays were used to detect herpesviruses: human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) and six periodontopathic anaerobic bacteria: Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Parvimonas micra, Treponema denticola, and Tannarella forsythia.

Results

Pericoronitis samples harbored HCMV and EBV at significantly higher rates than the control group (70 vs. 40 % and 46.7 vs. 15 %, P = 0.035, P = 0.021, respectively). P. micra and T. forsythia (66.7 vs. 0 %, and 40 vs. 10 %, P = 0.001, P = 0.021, respectively) were significantly more common in pericoronitis compared to the control group. Multivariate logistic regression analysis showed that the presence of T. forsythia was associated with pericoronitis development (OR 7.3, 95 % CI, 1.2–43.2, P = 0.028).

Conclusion

The occurrence of HCVM and EBV extends our previous knowledge on microbiota in pericoronitis. These PCR-based findings demonstrated that bacterial and viral DNA occurred concomitantly in pericoronitis samples. T. forsythia appeared to be significantly associated with pericoronitis development in the examined sample.

Clinical relevance

Herpesviral-bacterial co-infections might exacerbate the progression of pericoronitis.

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Acknowledgments

This study was supported by the Ministry of Education, Science and Technological Development of Republic of Serbia, Grant No. 175075.

Author information

Authors and Affiliations

  1. Clinic of Oral Surgery and Implantology, School of Dental Medicine, University of Belgrade, dr. Subotica 4, Belgrade, 11 000, Serbia

    Aleksandar Jakovljevic & Miroslav Andric

  2. Department of Virology, Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, dr. Subotica 1, Belgrade, 11 000, Serbia

    Aleksandra Knezevic

  3. Department of Medical Statistics and Informatics, School of Dental Medicine, University of Belgrade, Dr. Subotica 1, Belgrade, 11 000, Serbia

    Biljana Milicic

  4. Clinic of Restorative Dentistry and Endodontics, School of Dental Medicine, University of Belgrade, Rankeova 4, Belgrade, 11 000, Serbia

    Katarina Beljic-Ivanovic

  5. Clinic of Periodontology and Oral Medicine, School of Dental Medicine, University of Belgrade, Dr. Subotica 4, Belgrade, 11 000, Serbia

    Neda Perunovic

  6. Department of Biology and Human Genetics, School of Dental Medicine, University of Belgrade, Dr. Subotica 1, Belgrade, 11 000, Serbia

    Nadja Nikolic & Jelena Milasin

Authors
  1. Aleksandar Jakovljevic
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  2. Miroslav Andric
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  3. Aleksandra Knezevic
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  4. Biljana Milicic
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  5. Katarina Beljic-Ivanovic
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  6. Neda Perunovic
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  7. Nadja Nikolic
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  8. Jelena Milasin
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Corresponding author

Correspondence to Aleksandar Jakovljevic.

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Conflict of interest

The authors declare that they have no conflict of interest.

Funding

This study was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia, Grant No. 175075.

Ethical approval

This study has been approved by the Ethical Committee of the School of Dental Medicine, University of Belgrade (approval number: 36/27-2013), and has therefore been performed in accordance with the ethical standards laid down in 1964 Declaration of Helsinki and its later amendments.

Informed consent

All persons gave their written consent prior to their inclusion in the study.

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Jakovljevic, A., Andric, M., Knezevic, A. et al. Herpesviral-bacterial co-infection in mandibular third molar pericoronitis. Clin Oral Invest 21, 1639–1646 (2017). https://doi.org/10.1007/s00784-016-1955-4

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  • DOI: https://doi.org/10.1007/s00784-016-1955-4

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