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Enamel matrix derivative expedites osteogenic differentiation of BMSCs via Wnt/β-catenin pathway in high glucose microenvironment

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Abstract

Introduction

The influence of enamel matrix derivative (EMD) on proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) was explored in high glucose (HG) microenvironment with interaction of Wnt/β-catenin pathway.

Materials and Methods

Extraction of BMSCs from Sprague–Dawley rats, culture, and identification were manifested. The cells were treated with different concentration of EMD in HG to figure out the most available concentration for proliferation and osteogenic differentiation. Then, observation of cell growth curve and cell cycle changes, and detection of Osterix, runt-related transcription factor 2 (Runx2), COL-I, early osteogenic indexes, Calcium salt deposition, and β-catenin protein in Wnt/β-catenin pathway were assured. After adding Wnt/β-catenin pathway inhibitor (XAV-939) in the cells with osteogenesis induction, detection of binding of β-catenin to Osterix was clarified.

Results

Via identification BMSCs cultured in vitro was qualified. Different concentrations of EMD could accelerate cell proliferation in HG and osteogenesis induction, and 75 μg/mL EMD had the best effect. The HG augmented BMSCs proliferation and the propidium iodide index of flow cytometry cycle was elevated in HG, which were strengthened via the EMD. After BMSCs’ osteogenesis induction, Osterix, Runx2, CoL-1, early osteogenic indexes, and calcium salt deposition were reduced, but elevated via EMD. β-Catenin was the lowest in the HG, but elevated after EMD. After addition of XAV-939, reduction of β-catenin and the downstream (Osterix and Runx2) were manifested. Detection of binding protein bands was in β-catenin and Osterix of the HG after EMD treatment.

Conclusion

EMD may facilitate the osteogenic differentiation of BMSCs via activating the Wnt/β-catenin pathway in HG.

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Funding

1. National Natural Science Foundation of China (Grant No. 81860192) (http://nsfc.gov.cn/). 2. Guiyang Scientific and Technological Program [(2017)3040].

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Authors and Affiliations

  1. School of Stomatology, Guizhou Medical University, 9 Beijing Road, Yunyan District, Guiyang City, 550004, Guizhou Province, China

    MaoHua Meng, Ying Li, Xin Chen, QinYing Wang, JingQiao Chen, XingXing Xu, Huan Wang, JiaYu Shu, Jing Lu & Qiang Dong

  2. Department of Prosthodontics, Stomatological Hospital of Guizhou Medical University, 9 Beijing Road, Yunyan District, Guiyang City, 550004, Guizhou Province, China

    MaoHua Meng, Ying Li, Xin Chen, QinYing Wang, JingQiao Chen, XingXing Xu, Huan Wang, JiaYu Shu, Jing Lu & Qiang Dong

  3. Department of Preventive and Pediatric Dentistry, Stomatological Hospital of Guizhou Medical University, Guiyang City, 550004, Guizhou Province, China

    Qian Xia

  4. Clinical Research Center, The Affiliated Hospital of Guizhou Medical University, 9 Beijing Road, Yunyan District, Guiyang City, 550004, Guizhou Province, China

    ZhaoYang Ye

  5. Department of Prosthodontics, Guizhou Provincial People’s Hospital, 83 Zhongshan East Road, Guiyang, Guiyang City, 550003, Guizhou Province, China

    Bin Song

  6. Department of Stomatology, Guiyang Hospital of Stomatology, Guiyang City, 550005, Guizhou Province, China

    Lu Cheng

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  1. MaoHua Meng
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Correspondence to ZhaoYang Ye, Bin Song or Qiang Dong.

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Meng, M., Xia, Q., Li, Y. et al. Enamel matrix derivative expedites osteogenic differentiation of BMSCs via Wnt/β-catenin pathway in high glucose microenvironment. J Bone Miner Metab 40, 448–459 (2022). https://doi.org/10.1007/s00774-022-01318-6

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  • DOI: https://doi.org/10.1007/s00774-022-01318-6

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