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Agar-free high-performance thin-layer chromatography–bioautography method for the qualitative estimation of α-amylase inhibitor in Syzygium cumini seed extract and formulation

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Abstract

Quality control of herbal medicines is an important stage in generating effective and safe herbal remedies. A unique high-throughput approach for assessing anti-diabetic phytoconstituents in Syzygium cumini seed extract and marketed formulation was examined using high-performance thin-layer chromatography (HPTLC)–bioautography technology. Using β-sitosterol as reference, this agar-free bioautographic technique was devised and validated for the assessment of α-amylase inhibitors on TLC plates. The presence of some anti-diabetic compounds in plant samples was verified using a combination of biological detection and mass spectrometry (HPTLC–MS). The newly proposed HPTLC–bioautography screening method may be effective for evaluating the anti-diabetic activity of plant extracts and formulations. The proposed technique is quick, easy, precise, and dependable.

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Acknowledgements

We would like to thank the principal, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research Pimpri, Pune 411018, for providing facilities at our institute. Thanks are also due to the authorities of Savitribai Phule Pune University, Pune, for spectral studies.

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Authors and Affiliations

  1. Department of Pharmacognosy, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Sant. Tukaram Nagar Pimpri, Pune, Maharashtra, 411018, India

    S. S. Bhujbal

  2. Department of Quality Assurance, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune, 411018, India

    B. G. Chawale, M. A. Kale & R. V. Bhujbal

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  1. S. S. Bhujbal
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  2. B. G. Chawale
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  3. M. A. Kale
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  4. R. V. Bhujbal
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Correspondence to S. S. Bhujbal.

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Bhujbal, S.S., Chawale, B.G., Kale, M.A. et al. Agar-free high-performance thin-layer chromatography–bioautography method for the qualitative estimation of α-amylase inhibitor in Syzygium cumini seed extract and formulation. JPC-J Planar Chromat 35, 501–507 (2022). https://doi.org/10.1007/s00764-022-00205-0

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  • DOI: https://doi.org/10.1007/s00764-022-00205-0

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