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Stimulation quantification of four natural lipase inhibitors from Alismatis Rhizoma by high-performance thin-layer chromatography method

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Abstract

The lipase inhibitory activities of four main components from the rhizomes of Alisma orientale (Sam.) Juz. were evaluated by an in situ high-performance thin-layer chromatography (HPTLC)‒bioautographic assay taking orlistat as control standard. The order of relative activity was alisol B 23-acetate > alisol B > alisol A > alisol C 23-acetate. With that, an accurate, efficient and sustainable HPTLC method was developed to simultaneously determine the four lipase inhibitors from the methanolic extracts of Alismatis Rhizoma (AR). The method was carried out on HPTLC glassed plates (20 × 10 cm) coated with silica gel 60 F254 (0.2 mm thickness) using a mixture of cyclohexane and ethyl acetate (1:1, V/V) as the mobile phase. The RF values found for alisol B 23-acetate, alisol C 23-acetate, alisol B and alisol A were 0.62, 0.42, 0.28 and 0.09, respectively. The method was validated for specificity, linear range, precision, stability, and recovery. The results determined by scanning densitometry showed no significant difference to the results obtained by HPLC. The developed method was verified to be trustworthy for the evaluation of quality markers in AR.

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Acknowledgements

This study was financially supported by a project from Shanghai Natural Science Fund (No. 19ZR1457200) and a project from Chinese Pharmacopoeia Commission (No. 2018Z003).

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Authors and Affiliations

  1. Key Laboratory of Standardization of Chinese Medicines, Ministry of Education, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China

    Fei Yang, Mooseob Kim, Lihua Gu, Linnan Li, Li Yang & Zhengtao Wang

  2. Shanghai R&D Centre for Standardization of Chinese Medicines, Shanghai, China

    Mooseob Kim, Lihua Gu, Linnan Li, Li Yang & Zhengtao Wang

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  1. Fei Yang
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  2. Mooseob Kim
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  3. Lihua Gu
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  4. Linnan Li
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  5. Li Yang
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Correspondence to Lihua Gu or Zhengtao Wang.

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Yang, F., Kim, M., Gu, L. et al. Stimulation quantification of four natural lipase inhibitors from Alismatis Rhizoma by high-performance thin-layer chromatography method. JPC-J Planar Chromat 35, 3–12 (2022). https://doi.org/10.1007/s00764-022-00152-w

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  • DOI: https://doi.org/10.1007/s00764-022-00152-w

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