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Quality assessment of Sclerocarya birrea leaves and leaves products from Burkina Faso based on fingerprinting using HPTLC

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Abstract

The species Sclerocarya birrea, also known as Marula, is present in Sub-Saharan Africa. In Burkina Faso, the leaves are sold as a traditional improved medicine with antidiabetic indication. The objective of this study was to develop a simple, rapid and robust high-performance thin-layer chromatographic (HPTLC) method for the reliable quality control of S. birrea leaves in Burkina Faso and other West African countries. Chromatography was performed using HPTLC Si 60 F254 plates (20 × 10 cm) as the stationary phase and ethyl acetate‒formic acid‒water (80:10:10, V/V) as the mobile phase. Derivatization steps using natural product and anisaldehyde reagents were used after the development for the detections of flavonoids and triterpenoids. Detection was carried out prior to and after derivatization under UV 254 nm, 366 nm and white light. The HPTLC method was successfully applied to the analysis of 182 collected samples including different origins, related herbal drugs (different plant parts, confounding species) and 12 traditional improved medicines in different forms (capsule, powdered sample, tea). The method was then adapted for use in quality control at different levels in developing countries.

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Acknowledgements

The authors thank the Conservatoire et Jardin Botaniques de la ville de Genève (Dr. Louis Nusbaumer), the Actions Tenga Association (www.actionstenga.org), and Dr. Assane Diop for providing samples, and Dr. V. Dakuyo of Laboratoire Phytofla (www.laboratoiresphytofla.com) for providing Diabefla® products.

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Authors and Affiliations

  1. CAMAG Laboratory, Sonnenmattstrasse 11, 4132, Muttenz, Switzerland

    Thi Kieu Tiên Do & Eike Reich

  2. School of Pharmaceutical Sciences, University of Geneva, 1211, Geneva 4, Switzerland

    Kevin Clark & Philippe Christen

  3. Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1211, Geneva 4, Switzerland

    Kevin Clark & Philippe Christen

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  1. Thi Kieu Tiên Do
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  2. Kevin Clark
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  3. Philippe Christen
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  4. Eike Reich
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Correspondence to Thi Kieu Tiên Do.

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Do, T.K.T., Clark, K., Christen, P. et al. Quality assessment of Sclerocarya birrea leaves and leaves products from Burkina Faso based on fingerprinting using HPTLC. JPC-J Planar Chromat 33, 439–448 (2020). https://doi.org/10.1007/s00764-020-00058-5

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  • DOI: https://doi.org/10.1007/s00764-020-00058-5

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