Abstract
While several studies have implicated polyamines (PAs) in development, little research has been done in genetically tractable model systems like Drosophila. Here, we integrate transcriptional and metabolic data across Drosophila development, and are the first to show temporal, stage-specific regulation of PA accumulation in embryonic trachea and eye discs using immunohistochemistry. Understanding the regulation driving this accumulation can provide insight into PA metabolism and transport. Our findings suggest that Drosophila has great potential for investigating PAs in developmental biology.
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Abbreviations
- PA:
-
Polyamine
- Put:
-
Putrescine
- Spd:
-
Spermidine
- Spm:
-
Spermine
- Dpp:
-
Decapentaplegic
- Hh:
-
Hedgehog
- Wg:
-
Wingless
- EGFR:
-
Epidermal growth factor receptor
- ODC:
-
Ornithine decarboxylase
- SRM:
-
Spermine synthase
- SMS:
-
Spermidine synthase
- Arg:
-
Arginase
- SamDC:
-
S-adenosylmethionine decarboxylase
- SamS:
-
S-adenosylmethionine synthetase
- DHPS:
-
Deoxyhypusine synthase
- DOHH:
-
Deoxyhypusine hydroxylase
- SMO:
-
Spermine oxidase
- PAO:
-
Polyamine oxidase
- SAT1/2:
-
Diamine acetyltransferase 1/2
- AcCoA:
-
Acetyl coenzyme A
- OAZ/Oda:
-
Antizyme
- PCA:
-
Principle component analysis
- Btl:
-
Breathless
- E-Cad:
-
E-cadherin
- MF:
-
Morphogenetic furrow
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Acknowledgments
We are thankful for stocks from the Bloomington Drosophila Stock Center, NDIIF imaging facilities for confocal microscopy, and Iowa Hybridoma Bank for the Gasp antibody. We thank Erin Howe, Cody Narciso, Qinfeng Wu, and Pavel Brodskiy for critical reading of the manuscript. We are also grateful to Erica Smith, Gabrielle Dohmen, and Pavel Brodskiy for their early experimental work. This research was supported by the University of Notre Dame.
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Burnette, M., Zartman, J.J. Spatiotemporal patterning of polyamines in Drosophila development. Amino Acids 47, 2665–2670 (2015). https://doi.org/10.1007/s00726-015-2093-z
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DOI: https://doi.org/10.1007/s00726-015-2093-z