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BP8, a novel peptide from avian immune system, modulates B cell developments

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Abstracts

The bursa of Fabricius (BF) is the key humoral immune organ unique to birds, and is critical for early B-lymphocyte proliferation and differentiation. However, the molecular basis and mechanisms through which the BF regulates B cell development are not fully understood. In this study, we isolated and identified a new bursal peptide (BP8, AGHTKKAP) by RP-HPLC and MALDI-TOF–MS. BP8 promoted colony-forming pre-B formation, bound B cell precursor, regulated B cell development in vitro as well as in vivo, upstream of the EBF-E2A-Pax5 regulatory complex and increased immunoglobulin secretion. These data revealed a bursal-derived multifunctional factor BP8 as a novel biomaterial which is essential for the development of the immune system. This study elucidates further the mechanisms involved in humoral immune system and has implications in treating human diseases.

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Acknowledgments

This work was supported by the project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), the National Special Research Programs for Non-profit Trades, Ministry of Agriculture (No. 200803015) and international S&T Cooperation Program of China (ISTCP) (No. 2014DFR30980). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. We are grateful to the Life Sciences Institute of Nanjing Agricultural University for providing RP-HPLC facilities and to the Institute of Biochemistry and Cell Biology, SIBS, CAS for providing mass spectrometric analysis.

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The authors declare no competing financial interest.

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Authors and Affiliations

  1. Division of Key Lab of Animal Disease Diagnosis and Immunology of China’s Department of Agriculture, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Xiao-Dong Liu, Bin Zhou, Xiu-Li Feng, Rui-Bing Cao & Pu-Yan Chen

  2. College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, China

    Xiao-Dong Liu

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  1. Xiao-Dong Liu
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  2. Bin Zhou
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  3. Xiu-Li Feng
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  4. Rui-Bing Cao
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  5. Pu-Yan Chen
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Correspondence to Pu-Yan Chen.

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Liu, XD., Zhou, B., Feng, XL. et al. BP8, a novel peptide from avian immune system, modulates B cell developments. Amino Acids 46, 2705–2713 (2014). https://doi.org/10.1007/s00726-014-1824-x

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  • DOI: https://doi.org/10.1007/s00726-014-1824-x

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