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Transcriptome analysis of Citrus sinensis reveals potential responsive events triggered by Candidatus Liberibacter asiaticus

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Abstract

Citrus Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus (CLas), is a devastating immune-mediated disorder that has a detrimental effect on the citrus industry, with the distinguishing feature being an eruption of reactive oxygen species (ROS). This study explored the alterations in antioxidant enzyme activity, transcriptome, and RNA editing events of organelles in C. sinensis during CLas infection. Results indicated that there were fluctuations in the performance of antioxidant enzymes, such as ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR), peroxidase (POD), and superoxide dismutase (SOD), in plants affected by HLB. Transcriptome analysis revealed 3604 genes with altered expression patterns between CLas-infected and healthy samples, including those associated with photosynthesis, biotic interactions, and phytohormones. Samples infected with CLas showed a decrease in the expression of most genes associated with photosynthesis and gibberellin metabolism. It was discovered that RNA editing frequency and the expression level of various genes in the chloroplast and mitochondrion genomes were affected by CLas infection. Our findings provide insights into the inhibition of photosynthesis, gibberellin metabolism, and antioxidant enzymes during CLas infection in C. sinensis.

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Data availability

The raw reads for this project have been submitted to the Sequence Read Archive with the accession numbers SRR24101860, SRR24101859, SRR24101858, SRR24101857, SRR24101856, and SRR24101855.

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Acknowledgements

We thank academic editors from TopEdit for their help in grammar improvement.

Funding

This work was supported by the National Natural Science Foundation of China (32160621 and 32260659) and Innovation Fund Designated for Graduate Students of Jiangxi Province (YC2022-S928).

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  1. Chang Liu and Xiaopeng Chang contributed equally to this work.

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  1. College of Life Sciences, Gannan Normal University, Ganzhou, 341000, Jiangxi, China

    Chang Liu, Xiaopeng Chang, Fuxuan Li, Yana Yan, Xiru Zuo, Guiyan Huang & Ruimin Li

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Contributions

CL and XC participated in the design of the experiments, prepared RNA for sequencing, analyzed the data, and drafted the manuscript; FL, YY, and XZ participated in the real-time-quantitative PCR (RT-qPCR) validation and statistical analyses. GH and RL designed the experiments, analyzed the data, and drafted the manuscript.

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Correspondence to Guiyan Huang or Ruimin Li.

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709_2023_1911_MOESM1_ESM.tif

Supplementary file1 (TIF 1269 kb) Figure S1. Analysis of differentially expressed genes in the mitochondrion genome. (A) Volcano map analysis, (B) Heatmap analysis, (C) MapMan analysis.

709_2023_1911_MOESM2_ESM.tif

Supplementary file2 (TIF 1454 kb) Figure S2. RNA editing events observed in genes coded in the mitochondrion genome. (A) Venn diagram analysis, (B) Example genes with RNA editing events.

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Liu, C., Chang, X., Li, F. et al. Transcriptome analysis of Citrus sinensis reveals potential responsive events triggered by Candidatus Liberibacter asiaticus. Protoplasma 261, 499–512 (2024). https://doi.org/10.1007/s00709-023-01911-0

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