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The durum wheat plasma membrane Na+/H+ antiporter SOS1 is involved in oxidative stress response

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Abstract

We have shown previously that the durum wheat TdSOS1 excludes Na+ and Li+ ions outside cells. Moreover, this protein is activated by Arabidopsis kinase SOS2 through phosphorylation. The elimination of both SOS2 phosphorylation sites and the auto-inhibitory domain produces a hyperactive TdSOS1∆972 form, which have a maximal activity independent from the regulatory SOS2/SOS3 complex. We demonstrated that the expression of TdSOS1 enhances salt tolerance of the transgenic Arabidopsis plants. In this study, we analyzed the response to H2O2-induced oxidative stress of the transgenic Arabidopsis expressing one of the two TdSOS1 forms. Firstly, we showed that the exogenous H2O2 treatment leads to an accumulation of SOS1 transcripts in leaves and roots of the durum wheat and also in the transgenic plants. These transgenic plants showed significant oxidative stress tolerance compared to control plants, especially the plants expressing the hyperactive form. This tolerance was manifested by high proline accumulation and low malonyldialdehyde (MDA), O2˙− and H2O2 contents. Furthermore, the activities of three essential ROS scavenging enzymes (SOD, CAT, and POD) were higher in the transgenic plants under oxidative stress, as compared to control plants. Taken together, these data suggested that TdSOS1 plays a crucial role in response to oxidative stress.

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Acknowledgments

This study was supported by a grant from the Ministry of Higher Education, Scientific Research and Information Technology and Communication of Tunisia.

Author contributions

KF and FB designed the experiments, KF and ST performed the experiments, KF drafted the manuscript, and KM and FB critically revised the manuscript for important intellectual content and provided the final approval of the manuscript for publication.

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  1. Khaled Masmoudi

    Present address: International center for Biosaline Agriculture (ICBA), P.O. Box 14660, Dubai, UAE

Authors and Affiliations

  1. Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax (CBS)/University of Sfax, Route Sidi Mansour Km 6, B.P’1177’, 3018, Sfax, Tunisia

    Kaouthar Feki, Sana Tounsi, Khaled Masmoudi & Faiçal Brini

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  1. Kaouthar Feki
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  2. Sana Tounsi
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Correspondence to Faiçal Brini.

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Feki, K., Tounsi, S., Masmoudi, K. et al. The durum wheat plasma membrane Na+/H+ antiporter SOS1 is involved in oxidative stress response. Protoplasma 254, 1725–1734 (2017). https://doi.org/10.1007/s00709-016-1066-8

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  • DOI: https://doi.org/10.1007/s00709-016-1066-8

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