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Histone acetylation and reactive oxygen species are involved in the preprophase arrest induced by sodium butyrate in maize roots

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Abstract

Histone acetylation plays a critical role in controlling chromatin structure, and reactive oxygen species (ROS) are involved in cell cycle progression. To study the relationship between histone acetylation and cell cycle progression in plants, sodium butyrate (NaB), a histone deacetylase (HDAC) inhibitor that can cause a significant increase in histone acetylation in both mammal and plant genomes, was applied to treat maize seedlings. The results showed that NaB had significant inhibition effects on different root zones at the tissue level and caused cell cycle arrest at preprophase in the root meristem zones. This effect was accompanied by a dramatic increase in the total level of acetylated lysine 9 on histone H3 (H3K9ac) and acetylated lysine 5 on histone H4 (H4K5ac). The exposure of maize roots in NaB led to a continuous rise of intracellular ROS concentration, accompanied by a higher electrolyte leakage ratio and malondialdehyde (MDA) relative value. The NaB-treated group displayed negative results in both TdT-mediated dUTP nick end labelling (TUNEL) and γ-H2AX immunostaining assays. The expression of topoisomerase genes was reduced after treatment with NaB. These results suggested that NaB increased the levels of H3K9ac and H4K5ac and could cause preprophase arrest accompanied with ROS formation leading to the inhibition of DNA topoisomerase.

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Abbreviations

NaB:

Sodium butyrate

BSA:

Albumin from bovine serum

PBS:

Phosphate-buffered saline

ROS:

Reactive oxygen species

MDA:

Malondialdehyde

TUNEL:

TdT-mediated dUTP nick end labelling

H3K9ac:

Acetylated lysine 9 on histone H3

H4K5ac:

Acetylated lysine 5 on histone H4

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Acknowledgments

This work was supported by the NSFC (No. 31571265 and 31401706 ).

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Authors and Affiliations

  1. State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China

    Qi Zhang, Pu Wang, Haoli Hou, Hao Zhang, Junjun Tan, Yan Huang, Yingnan Li & Lijia Li

  2. Synergy Innovation Center of Biological Peptide Antidiabetics of Hubei Province, Engineering Technology Research Center of Biological Peptide Antidiabetics of Hubei Province, Department of Pharmaceutical Engineering, School of Life Science, Wuchang University of Technology, Wuhan, China

    Junjun Tan

  3. Industrial Crops Institute of Hubei Academy of Agricultural Sciences, No. 43, Nanhu Road, Hongshan District, Wuhan City, Hubei Province, China

    Jinping Wu & Zhengming Qiu

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  1. Qi Zhang
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  2. Pu Wang
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  3. Haoli Hou
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  4. Hao Zhang
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  5. Junjun Tan
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  6. Yan Huang
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  9. Zhengming Qiu
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  10. Lijia Li
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Correspondence to Lijia Li.

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Zhang, Q., Wang, P., Hou, H. et al. Histone acetylation and reactive oxygen species are involved in the preprophase arrest induced by sodium butyrate in maize roots. Protoplasma 254, 167–179 (2017). https://doi.org/10.1007/s00709-015-0928-9

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  • DOI: https://doi.org/10.1007/s00709-015-0928-9

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