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Complete genome sequence of a novel totivirus isolated from the leaves of Myrica rubra

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Abstract

Chinese bayberry is a fruit that is appreciated for its taste. A novel totivirus associated with rolling, disfiguring, chlorotic and vein-clearing symptoms on the leaf apices of Chinese bayberry was identified by transcriptome sequencing and reverse transcription PCR (RT-PCR). The complete genome of the virus was determined to be 4959 nucleotides long, and it contains two open reading frames (ORFs). Its genomic organization is similar to that of previously reported totiviruses. ORF1 encodes a putative coat protein (CP) of 765 aa, and ORF2 encodes an RNA-dependent RNA polymerase (RdRp) of 815 aa. These two putative proteins share 55.1% and 62.6%, amino acid sequence identity, respectively, with the corresponding proteins of Panax notoginseng virus A, respectively. According to the demarcation criteria for totivirus species established by the International Committee on Taxonomy of Viruses (ICTV), the new virus should be considered a member of a new species in the genus totivirus, family Orthototiviridae, which we have tentatively named ‘‘Myrica rubra-associated totivirus’’ (MRaTV).

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Data availability

The dataset generated in the current study is available in the GenBank database (accession no. OR08807).

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Funding

This research was funded by Yunnan Provincial Science and Technology Department-Applied Basic Research Joint Special Funds of Yunnan University of Chinese Medicine (202101AZ070001-011) and High-Level Talents Project of Yunnan University of Chinese Medicine (2019YZG09).

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Authors and Affiliations

  1. Institute of Medicinal Plant Cultivation, School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Resource, Yunnan University of Chinese Medicine, Kunming, 650500, Yunnan, China

    Weihao Luo, Lei Zhang, Xian Dong, Pengzhang Ji & Jiahong Dong

  2. Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650205, Yunnan, China

    Lizhen Zhang & Kuo Wu

Authors
  1. Weihao Luo
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  2. Lizhen Zhang
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  3. Lei Zhang
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  4. Xian Dong
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  5. Pengzhang Ji
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  6. Kuo Wu
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  7. Jiahong Dong
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Contributions

WL and JD conceived and designed the experiments. PJ and JD collected the samples. KW, LZ, and WL performed the experiments. LZ and XD analyzed the data. WL and JD wrote the paper. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Kuo Wu or Jiahong Dong.

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Luo, W., Zhang, L., Zhang, L. et al. Complete genome sequence of a novel totivirus isolated from the leaves of Myrica rubra. Arch Virol 169, 123 (2024). https://doi.org/10.1007/s00705-024-06048-w

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