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Current trends and new approaches for human norovirus replication in cell culture: a literature review

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Abstract

Human norovirus (HuNoV) is one of the world’s leading causes of acute gastroenteritis. At present, effective reproduction of the virus in cell cultures remains a challenge for virologists, as there is a lack of a permissive cell line that allows the entire viral life cycle to be reproduced. This is a barrier to the study of the HuNoV life cycle, its tropism, and virus-host interactions. It is also a major hurdle for the development of viral detection platforms, and ultimately for the development of therapeutics. The lack of an inexpensive, technically simple, and easily implemented cultivation method also negatively affects our ability to evaluate the efficacy of a variety of control measures (disinfectants, food processes) for human norovirus. In the process of monitoring this pathogen, it is necessary to detect infectious viral particles in water, food, and other environmental samples. Therefore, improvement of in vitro replication of HuNoV is still needed. In this review, we discuss current trends and new approaches to HuNoV replication in cell culture. We highlight ways in which previous research on HuNoV and other noroviruses has guided and influenced the development of new HuNoV culture systems and discuss the improvement of in vitro replication of HuNoV.

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Funding

This study was funded by the Russian Federal Service for Surveillance on Consumer Rights Protection and Human Well-being (scientific study no. 123051100045-0).

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Authors and Affiliations

  1. Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Federal Scientific Research Institute of Viral Infections «Virome», Ekaterinburg, 620030, Russian Federation

    Valentin V. Wasielewski, Tarek M. Itani & Aleksandr V. Semenov

  2. Institute of Disinfectology of the F.F. Erisman Federal Scientific Centre of Hygiene Rospotrebnadzor, Mosсow, Russian Federation

    Yuliya A. Zakharova

  3. Ural Federal University named after the First President of Russia B.N. Yeltsin, Ekaterinburg, Russian Federation

    Aleksandr V. Semenov

Authors
  1. Valentin V. Wasielewski
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  2. Tarek M. Itani
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  3. Yuliya A. Zakharova
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  4. Aleksandr V. Semenov
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Contributions

Wasielewski V.V.: database search, data analysis and interpretation, text drafting. Itani T.M.: text drafting, academic advising, and editing. Zakharova Yu. A.: academic advising and editing. Semenov A.V.: the concept of the review and editing. All authors read and approved the final draft.

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Correspondence to Tarek M. Itani.

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The authors declare that they have no known competing financial interests or personal relationships that could appear to influence the work reported in this paper.

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Communicated by Martin Chan

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Wasielewski, V.V., Itani, T.M., Zakharova, Y.A. et al. Current trends and new approaches for human norovirus replication in cell culture: a literature review. Arch Virol 169, 71 (2024). https://doi.org/10.1007/s00705-024-05999-4

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