Skip to main content
SpringerLink
Log in

A novel narnavirus isolated from Colletotrichum curcumae strain 780-2T

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

The complete genome of a novel mycovirus, Colletotrichum curcumae narnavirus 1 (CcNV1), derived from the phytopathogenic fungus Colletotrichum curcumae strain 780-2T, was sequenced and analyzed. The full sequence of CcNV1 is 3,374 nucleotides in length and contains a single large open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp) of 1,087 amino acids with a molecular mass of 124.2 kDa that shares the closest similarity with that of Monilinia narnavirus H (53.02% identity). RdRp phylogeny analysis showed that CcNV1 is a new member of the proposed genus "Betanarnavirus" within the family Narnaviridae. This is the first report of a novel narnavirus infecting the phytopathogenic fungus C. curcumae, the causal agent of leaf blight of Curcuma wenyujin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Ghabrial SA, Jiang D, Nibert ML, Suzuki N (2015) 50-plus years of fungal viruses. Virology 479:356–368

    Article  PubMed  Google Scholar 

  2. Ghabrial SA, Suzuki N (2009) Viruses of plant pathogenic fungi. Annu Rev Phytopathol 47(1):353–384

    Article  CAS  PubMed  Google Scholar 

  3. Pearson MN, Beever RE, Boine B, Arthur K (2009) Mycoviruses of filamentous fungi and their relevance to plant pathology. Mol Plant Pathol 10:115–128

    Article  CAS  PubMed  Google Scholar 

  4. Xie J, Jiang D (2014) New insights into mycoviruses and exploration for the biological control of crop fungal diseases. Annu Rev Phytopathol 52(1):45–68

    Article  CAS  PubMed  Google Scholar 

  5. Ghabrial SA, Suzuki N (2009) Viruses of plant pathogenic fungi. Annu Rev Phytopathol 47:353–384

    Article  CAS  PubMed  Google Scholar 

  6. Suzuki A, Kobayashi F, Abe M, Uchiumi T, Higashi S (2001) Cloning and expression of a down-regulated gene (TrEnodDR1) of white clover responded by the nod genes derived from Rhizobium leguminosarum bv. trifolii strain 4S. Gene 266(1–2):77–84

    Article  CAS  PubMed  Google Scholar 

  7. Kanematsu S, Sasaki A, Onoue M, Oikawa Y, Ito T (2010) Extending the fungal host range of a partitivirus and a mycoreovirus from Rosellinia necatrix by inoculation of protoplasts with virus particles. Phytopathology 100(9):922–930

    Article  CAS  PubMed  Google Scholar 

  8. Hillman BI, Cai G (2013) The family Narnaviridae: simplest of RNA viruses. Adv Virus Res 86:149–176

    Article  PubMed  Google Scholar 

  9. Wickner RB, Fujimura T, Esteban R (2013) Viruses and prions of Saccharomyces cerevisiae. Adv Virus Res 86:1–36

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Vainio EJ (2019) Mitoviruses in the conifer root rot pathogens Heterobasidion annosum and H. parviporum. Virus Res 271:1–5

    Article  Google Scholar 

  11. Ong JWL, Li H, Sivasithamparam K, Dixon KW, Jones MGK, Wylie SJ (2016) Novel Endorna-like viruses, including three with two open reading frames, challenge the membership criteria and taxonomy of the Endornaviridae. Virology 499:203–211

    Article  CAS  PubMed  Google Scholar 

  12. Zhang R, Hisano S, Tani A, Kondo H, Kanematsu S, Suzuki N (2016) A capsidless ssRNA virus hosted by an unrelated dsRNA virus. Nat Microbiol 1:15001

    Article  CAS  PubMed  Google Scholar 

  13. Jia H, Dong K, Zhou L, Wang G, Hong N, Jiang D (2017) A dsRNA virus with filamentous viral particles. Nat Commun 8:168

    Article  PubMed  PubMed Central  Google Scholar 

  14. Wickner RB, Fujimura T, Esteban R (2013) Viruses and prions of Saccharomyces cerevisiae. Adv Virus Res 86:1–36

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Klumpp K, Ruigrok RW, Baudin F (1997) Roles of the influenza virus polymerase and nucleoprotein in forming a functional RNP structure. EMBO J 16(6):1248–1257

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Area E, Martín-Benito J, Gastaminza P, Torreira E, Valpuesta JM, Carrascosa JL, Ortín J (2004) 3D structure of the influenza virus polymerase complex: localization of subunit domains. Proc Natl Acad Sci USA 101(1):308–313

    Article  CAS  PubMed  Google Scholar 

  17. Göertz GP, Miesen P, Overheul GJ, Van Rij RP, Van Oers MM, Pijlman GP (2019) Mosquito small RNA responses to West Nile and insect-specific virus infections in Aedes and Culex mosquito cells. Viruses 11(3):E271

    Article  Google Scholar 

  18. Wang Y, Liu S, Zhu HJ, Zhong J (2019) Molecular characterization of a novel mycovirus from the plant pathogenic fungus Colletotrichum gloeosporioides. Arch Virol 164(11):2859–2863

    Article  CAS  PubMed  Google Scholar 

  19. Xu X, Hai D, Li J, Huang F, Wang Y (2022) Molecular characterization of a novel penoulivirus from the phytopathogenic fungus Colletotrichum camelliae. Arch Virol 167(2):641–644

    Article  CAS  PubMed  Google Scholar 

  20. Cao J, Xie F, Zhang Z, Zhu H, Zhou X (2022) Molecular characterization of a novel polymycovirus identified in the phytopathogenic fungus Colletotrichum gloeosporioides. Arch Virol 167(12):2805–2810

    Article  CAS  PubMed  Google Scholar 

  21. Guo J, Zhu JZ, Zhou XY, Zhong J, Li CH, Zhang ZG, Zhu HJ (2019) A novel ourmia-like mycovirus isolated from the plant pathogenic fungus Colletotrichum gloeosporioides. Arch Virol 164(10):2631–2635

    Article  CAS  PubMed  Google Scholar 

  22. Wang Y, Liu S, Zhu HJ, Zhong J (2019) Molecular characterization of a novel mycovirus from the plant pathogenic fungus Colletotrichum gloeosporioides. Arch Virol 164(11):2859–2863

    Article  CAS  PubMed  Google Scholar 

  23. Zhong J, Chen D, Lei XH, Zhu HJ, Zhu JZ, Gao BD (2014) Detection and characterization of a novel Gammapartitivirus in the phytopathogenic fungus Colletotrichum acutatum strain HNZJ001. Virus Res 190:104–109

    Article  CAS  PubMed  Google Scholar 

  24. Jia H, Dong K, Zhou L, Wang GP, Hong N, Jiang DH, Xu WX (2017) A dsRNA virus with filamentous viral particles. Nat Commun 8(1):168

    Article  PubMed  PubMed Central  Google Scholar 

  25. Zhai L, Zhang M, Hong N, Hong N, Xiao F, Fu M, Xiang J, Wang G (2018) Identification and characterization of a novel hepta-segmented dsRNA virus from the phytopathogenic fungus Colletotrichum fructicola. Front Microbiol 9:754

    Article  PubMed  PubMed Central  Google Scholar 

  26. Wang H, Liu H, Zhou Q (2021) The complete genome sequence of a new mitovirus from the phytopathogenic fungus Colletotrichum higginsianum. Arch Virol 166(5):1481–1484

    Article  CAS  PubMed  Google Scholar 

  27. Liu H, Wang H, Lu X, Xiao C, Peng B, Zhou Q (2021) Molecular characterization of a novel single-stranded RNA virus, ChRV1, isolated from the plant-pathogenic fungus Colletotrichum higginsianum. Arch Virol 166(6):1805–1809

    Article  CAS  PubMed  Google Scholar 

  28. Lima JS, Figueiredo JG, Gomes RG, Stringari D, Goulin EH, Adamoski D, Kava-Cordeiro V, Galli-Terasawa LV, Glienke C (2012) Genetic Diversity of Colletotrichum spp. an Endophytic Fungi in a Medicinal Plant. Brazilian Pepper Tree. Isrn Microbiology,215716

  29. Komatsu K, Katayama Y, Omatsu T, Mizutani T, Fukuhara T, Kodama M, Arie T, Teraoka T, Moriyama H (2016) Genome sequence of a novel mitovirus identified in the phytopathogenic fungus Alternaria arborescens. Arch Virol 161:2627–2631

    Article  CAS  PubMed  Google Scholar 

  30. Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23(21):2947–2948

    Article  CAS  PubMed  Google Scholar 

  32. Kamer G, Argos P (1984) Primary structural comparison of RNA-dependent RNA polymerase from plant, animal, and bacterial viruses. Nucleic Acid Res 12(18):7269–7282

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Koonin EV, Choi GH, Nuss DL, Shapira R, Carrington JC (1991) Evidence for common ancestry of a chestnut blight hypovirulence associated double-stranded RNA and a group of positive-strand RNA plant viruses. Proc Natl Acad Sci USA 88:10647–10651

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Ng KK, Arnold JJ, Cameron CE (2008) Structure-function relationships among RNA-dependent RNA polymerases. Curr Top Microbiol Immunol 320(1):137–156

    CAS  PubMed  PubMed Central  Google Scholar 

Download references

Funding

This study was supported by the National Natural Science Foundation of China (no. 32260648), the Hainan Province Key R&D Project (no. ZDYF2022XDNY242), and the Scientific Research Foundation for Advanced Talents, Hainan University (no. KYQD(ZR)1873).

Author information

Authors and Affiliations

  1. Hainan Yazhou Bay Seed Laboratory, Sanya Nanfan Research Institute of Hainan University, 572025, Sanya, China

    Yujia Fu, Tian Wang, Siyu Zhou, Jingyi Zhou, Yang Zhao, Daipeng Chen & Li Zheng

  2. Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, School of Plant Protection, Ministry of Education, Hainan University, 570228, Haikou, Hainan, China

    Yujia Fu, Tian Wang, Siyu Zhou, Jingyi Zhou, Yang Zhao, Daipeng Chen & Li Zheng

Authors
  1. Yujia Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tian Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Siyu Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jingyi Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yang Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Daipeng Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Li Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Li Zheng.

Ethics declarations

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with animals or human participants performed by any of the authors.

Additional information

Communicated by Ioly Kotta-Loizou.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Yujia Fu and Tian Wang contributed equally to this work.

Electronic Supplementary Material

Below is the link to the electronic supplementary material

Supplementary Material 1

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Fu, Y., Wang, T., Zhou, S. et al. A novel narnavirus isolated from Colletotrichum curcumae strain 780-2T. Arch Virol 168, 226 (2023). https://doi.org/10.1007/s00705-023-05847-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00705-023-05847-x

Search

Navigation