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Effect of NV gene deletion in the genome of hirame rhabdovirus (HIRRV) on viral replication and the type I interferon response of the host cell

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Abstract

Hirame rhabdovirus (HIRRV), a member of the genus Novirhabdovirus, causes morbidity and mortality in farmed olive flounder (Paralichthys olivaceus). As no information is available on the role of the NV gene of HIRRV, we produced a recombinant HIRRV with the NV gene deleted (rHIRRV-ΔNV) using reverse genetic technology and investigated whether the NV gene knockout affected HIRRV replication and the type I interferon response of the host cell. The rescue of rHIRRV-ΔNV was successful only when IRF9-gene-knockout Epithelioma papulosum cyprini (ΔIRF9-EPC) cells were used, suggesting that the NV protein of HIRRV might be involved in inhibition of the type I interferon response of the host cell. This conclusion was also supported by the significantly higher level of Mx gene induction in EPC cells infected with rHIRRV-ΔNV than in cells infected with recombinant HIRRV without the deletion. When cells were coinfected with rHIRRV-ΔNV and either wild-type HIRRV or wild-type viral hemorrhagic septicemia virus (VHSV), there was a decrease in the growth rate of not only wild-type HIRRV but also wild-type VHSV in a concentration-dependent manner. Further studies are required to investigate the role of HIRRV NV in virulence and its possible importance for the development of attenuated vaccines.

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Acknowledgements

This research was a part of the project (2020) titled ‘Development of rapid and sensitive diagnostic methods for the quarantine of aquatic animals and their products’, funded by the Ministry of Oceans and Fisheries, Korea.

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Authors and Affiliations

  1. Department of Aquatic Life Medicine, Pukyong National University, Busan, 48513, South Korea

    Su Jeong Ryu, Jun Soung Kwak & Ki Hong Kim

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  1. Su Jeong Ryu
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  2. Jun Soung Kwak
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  3. Ki Hong Kim
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Correspondence to Ki Hong Kim.

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No part of this study included experiments with human participants or animals performed by any of the authors.

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Ryu, S.J., Kwak, J.S. & Kim, K.H. Effect of NV gene deletion in the genome of hirame rhabdovirus (HIRRV) on viral replication and the type I interferon response of the host cell. Arch Virol 167, 77–84 (2022). https://doi.org/10.1007/s00705-021-05286-6

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  • DOI: https://doi.org/10.1007/s00705-021-05286-6

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