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Characterization of a novel Vibrio parahaemolyticus host-phage pair and antibacterial effect against the host

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Abstract

Vibrio parahaemolyticus is a widely recognized pathogen that has caused numerous outbreaks and is prevalent in the marine environment. In this study, we investigated the characteristics of the novel V. parahaemolyticus strain BTXS2 and its associated phage, VB_VpP_BT-1011, isolated from the Bohai Coast (Tianjin, China). Strain BTXS2 is a short coryneform bacterium with a terminal flagellum and is able to utilize and metabolize a wide variety of organic matter because of its unique carbon source utilization and enzyme activity. It grows well in medium between pH 5.0 and 9.0 and salinities of simulated freshwater, estuary water, and seawater (NaCl 0.5%-3%). Multiple antibiotic resistance genes and virulence genes that endanger human health were found in the BTXS2 genome. Phage VB_VpP_BT-1011, which infects BTXS2, is a 40,065-bp double-stranded DNA virus of the family Myoviridae with a latent time of 30 min and burst size of 24 PFU/cell. Like its host, the phage tolerates a broad range of environmental conditions (salinity, 0-3% NaCl; pH 5.0-9.0; temperature, 4-37°C). A host range test showed that the phage only infected and inhibited isolate BTXS2. In summary, we investigated a novel V. parahaemolyticus host-phage pair and the antibacterial effect of the phage on V. parahaemolyticus, providing insights into marine microbial ecology and risks.

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Availability of data and materials

The nucleotide sequence of V. parahaemolyticus BTXS2 can be viewed in the NCBI database (https://www.ncbi.nlm.nih.gov/nuccore/?term=BTXS2), and the nucleotide sequence of the phage can be viewed at https://www.ncbi.nlm.nih.gov/nuccore/MW009675.1.

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Acknowledgments

This work was supported by the National Key R&D Program of China (grant number 2018YFD0800104), the Special Fund of China (grant number AWS18J004 and 2019-JCJQ-JJ-163), the Natural Science Foundation of Tianjin, China (grant number 17JCZDJC39100 and 19JCYBJC23800), and the National Natural Science Foundation of China (grant number 51808468). We thank Dan Huang for her help in analyzing the genetic information and function of phage, and Kun He, Yanping Yang, and Chunchun Zhang for their assistance in conducting host growth and phage experiments.

Funding

This research was funded by the National Key R&D Program of China (Grant number 2018YFD0800104), the Special Fund of China (Grant number AWS18J004 and 2019-JCJQ-JJ-163), the Natural Science Foundation of Tianjin, China (Grant number 17JCZDJC39100 and 19JCYBJC23800), and the National Natural Science Foundation of China (Grant number 51808468).

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Author notes

  1. Chao Gao and Xiaobo Yang have contributed equally to this work.

Authors and Affiliations

  1. School of Marine Science and Technology, Tianjin University, Tianjin, 300072, China

    Chao Gao & Zhiguang Niu

  2. Tianjin Institute of Environmental and Operational Medicine, Tianjin, 300050, China

    Chao Gao, Xiaobo Yang, Chen Zhao, Chenyu Li, Shang Wang, Xi Zhang, Bin Xue, Zhuosong Cao, Hongrui Zhou, Yutong Yang, Zhiqiang Shen, Jingfeng Wang & Zhigang Qiu

  3. School of Environmental and Chemical Engineering, Xi’an Polytechnic University, Xi’an, 710600, Shanxi, China

    Zhuosong Cao

  4. Department of Civil and Environmental Engineering, Rice University, Houston, TX, 77005, USA

    Pingfeng Yu

  5. College of Chemistry and Chemical Engineering, Southwest Petroleum University, Chengdu, 610500, China

    Lingli Li

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  1. Chao Gao
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  2. Xiaobo Yang
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  3. Chen Zhao
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Contributions

Conceptualization: CG, XY, C Z, CL, SW, XZ, BX, ZS, JW, LL, PY, ZN, and ZQ. Data curation: CG, XY, ZN, and ZQ. Formal analysis: CG, XY, CZ, SW, ZC, HZ, YY, ZN, and ZQ. Funding acquisition: SW, JW, LL, and ZQ. Investigation: CG, XY, CL, SW, XZ, BX, ZS, JW, ZN, and ZQ. Methodology: CG, XY, CZ, SW, XZ, BX, ZS, JW, PY, ZN, and ZQ. Project administration: JW, ZN, and ZQ. Resources: JW and ZQ. Validation: CG, XY, CZ, SW, ZC, HZ, YY, ZN, and ZQ. Writing—original draft: CG, XY, and ZQ. Writing—review and editing: CG, XY, PY, and ZQ. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Jingfeng Wang, Lingli Li, Zhiguang Niu or Zhigang Qiu.

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Gao, C., Yang, X., Zhao, C. et al. Characterization of a novel Vibrio parahaemolyticus host-phage pair and antibacterial effect against the host. Arch Virol 167, 531–544 (2022). https://doi.org/10.1007/s00705-021-05278-6

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