Skip to main content
SpringerLink
Log in

Complete genome sequence of a previously undescribed monopartite begomovirus and betasatellite infecting Malvastrum coromandelianum in Cambodia

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

A previously undescribed monopartite begomovirus was identified in Kampot province, Cambodia, in Malvastrum coromandelianum plants exhibiting yellow vein symptoms characteristic of begomovirus infections. The apparently full-length viral component was cloned and sequenced following enrichment of circular DNA by rolling-circle amplification and restriction enzyme digestion. The genome of the virus was 2737 nucleotides in length (KP188831) and exhibited an organization like that of other monopartite begomoviruses, sharing the highest nucleotide sequence similarity (87.7% identity) with ageratum yellow vein virus (AM940137). A satellite molecule was amplified from total DNA by PCR amplification, using the betasatellite-specific primer pair β01/β02. The satellite molecule (1346 nt, KP188832) had structural characteristics like those of other betasatellites associated with begomoviruses and shared the highest nucleotide sequence similarity (84.8% identity) with malvastrum yellow vein betasatellite (MN205547). According to the criteria established for species demarcation for classification of begomoviruses (family Geminiviridae) and betasatellites (family Tolecusatellitidae), respectively, the virus isolate from M. coromandelianum in Cambodia is a previously undescribed novel monopartite begomovirus, for which the name “malvastrum yellow vein Cambodia virus” (MaYVCV) is proposed, and the betasatellite is a previously undescribed novel betasatellite, for which the name “malvastrum yellow vein Cambodia betasatellite” (MaYVKHB) is proposed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Varsani A, Navas-Castillo J, Moriones E, Hernandezzepeda C, Idris AM, Brown JK, Zerbini FM, Martin DP (2014) Establishment of three new genera in the family geminiviridae: becurtovirus, eragrovirus and turncurtovirus. Arch Virol 159(8):2193–2203

    Article  CAS  Google Scholar 

  2. Varsani A, Roumagnac P, Fuchs M, Navascastillo J, Moriones E, Idris AM, Briddon RW, Rivere-Bustamante R, Zerbini FM, Martin DP (2017) Capulavirus and Grablovirus: two new genera in the family Geminiviridae. Arch Virol 162(6):1819–1831

    Article  CAS  Google Scholar 

  3. Navas-Castillo J, Fiallo-Olivé E, Sánchez-Campos S (2011) Emerging virus diseases transmitted by whiteflies. Annu Rev Phytopathol 49:219–248

    Article  CAS  Google Scholar 

  4. Hanley-Bowdoin L, Settlage SB, Orozco BM, Nagar S, Robert D (1999) Geminiviruses: models for plant DNA replication, transcription, and cell cycle regulation. Crit Rev Plant Sci 18:71–106

    Article  CAS  Google Scholar 

  5. Fiallo-Olivé E, Martínez-Zubiaur Y, Moriones E, Navas-Castillo J (2012) A novel class of DNA satellites associated with New World begomoviruses. Virology 426:1–6

    Article  Google Scholar 

  6. Lozano G, Trenado HP, Fiallo-Olivé E, Chirinos D, Geraud-Pouey F, Briddon RW, Navas-Castillo J (2016) Characterization of non-coding DNA satellites associated with sweepoviruses (genus Begomovirus, Geminiviridae)—definition of a distinct class of begomovirus-associated satellites. Front Microbiol 7:162

    Article  Google Scholar 

  7. Zhou XP (2013) Advances in understanding begomovirus satellites. Annu Rev Phytopathol 51:357–381

    Article  CAS  Google Scholar 

  8. Alabi OJ, Villegas C, Gregg L, Murray KD (2016) Complete nucleotide sequences of a new bipartite begomovirus from malvastrum sp. plants with bright yellow mosaic symptoms in south Texas. Arch Virol 161(6):1729–1733

    Article  CAS  Google Scholar 

  9. André PG, Martin DP, Roye ME (2010) Molecular characterization and phylogeny of two begomoviruses infecting Malvastrum americanumin Jamaica: evidence of the contribution of inter-species recombination to the evolution of malvaceous weed-associated begomoviruses from the northern Caribbean. Virus Genes 40(2):256–266

    Article  Google Scholar 

  10. Zhou X, Xie Y, Peng Y, Zhang Z (2003) Malvastrum yellow vein virus, a new begomovirus species associated with satellite DNA molecule. Chinese Sci Bull 48(20):2206–2210

    Article  Google Scholar 

  11. Jiang T, Liu P, Liao BL, Wu JX, Huang CJ (2010) Malvastrum yellow vein yunnan virus is a monopartite begomovirus. Acta Virol 54(1):21–26

    Article  CAS  Google Scholar 

  12. Huang JF, Zhou XP (2006) Molecular characterization of two distinct begomoviruses from Ageratum conyzoides and Malvastrum coromandelianum in China. J Phytopathol 154(11–12):648–653

    Article  CAS  Google Scholar 

  13. Rahman SU, Zubair M, Ahmed N, Khan MZ, Raza G, Amin I, Mansoor S (2019) Identification of “Malvastrum yellow vein Lahore virus” a proposed new species of begomovirus in association with cotton leaf curl Multan betasatellite infecting green bean (Phaseolus vulgaris) in Pakistan. Australasian Plant Dis Notes 14(1):1–3

    Article  CAS  Google Scholar 

  14. Guo X, Shi M, Zhou X (2007) Complete nucleotide sequences of Malvastrum yellow mosaic virus and its associated DNAβ molecule. Arch Virol 152(3):641–643

    Article  CAS  Google Scholar 

  15. Brown JK, Zerbini FM, Navas-Castillo J, Moriones E, Ramos-Sobrinho R, Silva JC, Fiallo-Olive E, Briddon RW, Hernandez-Zepeda C, Idris A, Malathi VG, Martin DP, Rivera-Bustamante R, Ueda S, Varsani A (2015) Revision of Begomovirus taxonomy based on pairwise sequence comparisons. Arch Virol 160:1593–1619

    Article  CAS  Google Scholar 

  16. Wyatt SD, Brown JK (1996) Detection of subgroup III geminivirus isolates in leaf extracts by degenerate primers and polymerase chain reaction. Phytopathology 86:1288–1293

    Article  Google Scholar 

  17. He ZF, Yu H, Luo FF (2004) Detection of whitefly-transmitted geminiviruses from tomato by PCR. Virol Sin 19:67–69

    CAS  Google Scholar 

  18. Rojas MR, Gilbertson RL, Russell DR, Maxwell DP (1993) Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant Dis 77:340–347

    Article  CAS  Google Scholar 

  19. Briddon RW, Bull SE, Mansoor S, Amin I, Markham PG (2002) Universal primers for the PCR-mediated amplification of DNA β. Mol Biotechnol 20:315–318

    Article  CAS  Google Scholar 

  20. Bull SE, Briddon RW, Markham PG (2003) Universal primers for the PCR-mediated amplification of DNA 1: a satellite-Like molecule associated with begomovirus-DNA beta complexes. Mol Biotechnol 23(1):83–86

    Article  CAS  Google Scholar 

  21. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    Article  CAS  Google Scholar 

  22. Muhire B, Varsani A, Martin DP (2014) SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. PLoS ONE 9:e108277

    Article  Google Scholar 

  23. Tamura K, Stecher G, Peterson DS, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30(12):2725–2729

    Article  CAS  Google Scholar 

  24. Martin DP, Murrell B, Golden M, Khoosal A, Muhire BM (2015) RDP4: Detection and analysis of recombination patterns in virus genomes. Virus Evol 1(1):vev003

    Article  Google Scholar 

  25. Briddon RW, Navas-Castillo J, Fiallo-Olivé E (2016) ICTV taxonomic proposal 2016.021a-kP.A.v2.Tolecusatellitidae. Create the Tolecusatellitidae, a new family of single-stranded DNA satellites with two genera

  26. Leke WN, Kvarnheden A, Avelar S, Brown JK (2020) Molecular characterization of two previously undescribed begomovirus-associated alphasatellite molecules infecting malvaceous species in Cameroon. Arch Virol 165:775–779

    Article  CAS  Google Scholar 

  27. Wu CZ, Zhou XP (2006) First report of malvastrum leaf curl virus infecting papaya. J Plant Pathol 88(3):342

    Google Scholar 

Download references

Acknowledgements

This study was funded by the Scientific and Technological Assistance Project for Developing Countries, China (KY201402015), the National Natural Science Foundation of China (31871937), Guangdong Basic and Applied Basic Research Foundation (2019A1515012150), the Science and Technology Program of Guangzhou, China (201904010173), and the Special Fund for Scientific Innovation Strategy-Construction of High-Level Academy of Agriculture Science (R2019PY-JX005).

Author information

Authors and Affiliations

  1. Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China

    Yafei Tang, Zhenggang Li, Xiaoman She, Lin Yu, Guobing Lan, Lihua Lv & Zifu He

  2. Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou, 510640, China

    Yafei Tang & Zifu He

  3. School of Plant Sciences, The University of Arizona, Tucson, AZ, 85721, USA

    Judith K. Brown

Authors
  1. Yafei Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhenggang Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiaoman She
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lin Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Guobing Lan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lihua Lv
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Judith K. Brown
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Zifu He
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zifu He.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This research did not use human participants or other animals.

Additional information

Handling Editor: Jesús Navas-Castillo.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tang, Y., Li, Z., She, X. et al. Complete genome sequence of a previously undescribed monopartite begomovirus and betasatellite infecting Malvastrum coromandelianum in Cambodia. Arch Virol 166, 1789–1793 (2021). https://doi.org/10.1007/s00705-021-05016-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-021-05016-y

Search

Navigation