Skip to main content

Advertisement

SpringerLink
Log in

Genetic characterization of a novel porcine reproductive and respiratory syndrome virus type I strain from southwest China

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically devastating viral diseases in the global pig industry. Recently, we isolated and plaque-purified porcine reproductive and respiratory syndrome virus (PRRSV) strain SC2020-1 from “aborted piglets” on a farm in Sichuan, China. To investigate the molecular biological characteristics of this strain, it was subjected to genome sequencing and analysis. The full-length genome sequence of strain SC2020-1 was 87.7% identical to that of the Lelystad strain (PRRSV type I protoype strain) and 82.2-84.8% identical to PRRSV type I isolates from China. NSP2, ORF3, and ORF4 were the most variable regions and contained discontinuous deletions or insertions when compared to other PRRSV type I strains. Phylogenetic analysis of the complete genome sequence showed that SC2020-1 clustered with PRRSV type I but outside of the three previously described branches (Lelystad virus-like, Amervac PRRS-like, and BJEU06-1-like). The Nsp2 gene was in the same branch with EUGDHD strains from China. This is the first report of PRRSV type I infection associated with abortion in sows in southwest China. Close attention should be paid to the prevention and control of this evolving virus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Data availability statement

All sequences generated in this study were submitted to the GenBank database under accession no. MW115431.

References

  1. Dokland T (2010) The structural biology of PRRSV. Virus Res 154(1–2):86–97

    Article  CAS  Google Scholar 

  2. Nilubol D, Platt KB, Halbur PG, Torremorell M, Harris DL (2004) The effect of a killed porcine reproductive and respiratory syndrome virus (PRRSV) vaccine treatment on virus shedding in previously PRRSV infected pigs. Vet Microbiol 102(1–2):11–18

    Article  CAS  Google Scholar 

  3. Hanada K, Suzuki Y, Nakane T, Hirose O, Gojobori TJMB (2005) The origin and evolution of porcine reproductive and respiratory syndrome viruses. Mol Biol Evol 22(4):1024–1031

    Article  CAS  Google Scholar 

  4. Snijder EJ, Tol HV, Roos N, Pedersen KW (2001) Non-structural proteins 2 and 3 interact to modify host cell membranes during the formation of the arterivirus replication complex. J Gen Virol 82(Pt 5):985–994

    Article  CAS  Google Scholar 

  5. Belák S, Storgaard T, Drew TW, Stadejek T, Oleksiewicz MB, Pejsak Z, Stankevicius A (2002) Identification of radically different variants of porcine reproductive and respiratory syndrome virus in Eastern Europe: towards a common ancestor for European and American viruses. J Gen Virol 83(8):1861–1873

    Article  Google Scholar 

  6. Chen J, Liu T, Zhu CG, Jin YF, Zhang YZ (2006) Genetic variation of Chinese PRRSV strains based on ORF5 sequence. J Biochem Genet 44(9–10):421–431

    Article  Google Scholar 

  7. Wensvoort G, Terpstra C, Pol JMA, Ter Laak EA, Bloemraad M, De Kluyver EP, Kragten C, Van Buiten L, Den Besten A, Wagenaar F (1991) Mystery swine disease in The Netherlands: the isolation of Lelystad virus. Vet Q 13(3):121–130

    Article  CAS  Google Scholar 

  8. Collins JE, Benfield DA, Christianson WT, Harris L, Hennings JC, Shaw DP, Goyal SM, McCullough S, Morrison RB, Joo HS, Gorcyca D, Chladek D (1992) Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs. J Vet Diagn Investig 4(2):117–126

    Article  CAS  Google Scholar 

  9. Jantafong T, Sangtong P, Saenglub W, Mungkundar C, Romlamduan N, Lekchareonsuk C, Lekcharoensuk P (2015) Genetic diversity of porcine reproductive and respiratory syndrome virus in Thailand and Southeast Asia from 2008 to 2013. Vet Microbiol 176(3–4):229–238

    Article  CAS  Google Scholar 

  10. Li B, Fang L, Liu S, Zhao F, Jiang Y, He K, Chen H, Xiao S (2010) The genomic diversity of Chinese porcine reproductive and respiratory syndrome virus isolates from 1996 to 2009. Vet Microbiol 146(3–4):226–237

    Article  CAS  Google Scholar 

  11. Li Y, Wang X, Ping J, Wang X, Wen C, Wang X, Wang K (2009) Genetic variation analysis of porcine reproductive and respiratory syndrome virus isolated in China from 2002 to 2007 based on ORF5. Vet Microbiol 138(1–2):150–155

    Article  CAS  Google Scholar 

  12. Liu JK, Wei CH, Yang XY, Hou XL, Dai AL (2013) Genetic diversity and evolutionary characterization of Chinese porcine reproductive and respiratory syndrome viruses based on NSP2 and ORF5. Arch Virol 158(8):1811–1816

    Article  CAS  Google Scholar 

  13. Lei Z, Wang Z, Ding Y, Ge X, Xin G, Yang H (2015) NADC30-like strain of porcine reproductive and respiratory syndrome virus, China. Emerg Infect Dis 21(12):2256–2257

    Article  Google Scholar 

  14. Zhang HL, Zhang WL, Xiang LR, Leng CL, Tian ZJ, Tang YD, Cai XH (2018) Emergence of novel porcine reproductive and respiratory syndrome viruses (ORF5 RFLP 1-7-4 viruses) in China. Vet Microbiol 222:105–108

    Article  CAS  Google Scholar 

  15. Chen N, Zhen C, Yu X, Deng X, Zhao T (2011) Emergence of novel European genotype porcine reproductive and respiratory syndrome virus in mainland China. J Gen Virol 92(4):880–892

    Article  CAS  Google Scholar 

  16. Zhi Zhou QL, Dongmei Hu, Zhang Q, Han T, Ma Y, Xiaoxue Gu, Zhai X, Tian K (2015) Complete genomic characterization and genetic diversity of four European genotype porcine reproductive and respiratory syndrome virus isolates from China in 2011. Virus Genes 51(3):375–384

    Article  Google Scholar 

  17. Liu JK, Wei CH, Dai AL, Fan KW, Yang BH, Huang CF, Li XH, Yang XY, Luo ML (2017) Complete genomic characterization of two European-genotype porcine reproductive and respiratory syndrome virus isolates in Fujian province of China. Arch Virol 162(3):823–833

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research was funded by the National Key Research and Development Program (2018YFD0500802), the Science and Technology Program of Sichuan Province (2018NZ0150), and the Sichuan Veterinary Medicine and Drug Innovation Group of the China Agricultural Research System (CARS-SVDIP).

Author information

Authors and Affiliations

  1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China

    Jun Zhao, Ling Zhu, Huidan Deng, Fengqing Li, Lei Xu, Xiangang Sun & Zhiwen Xu

  2. Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu, China

    Wenqi Yin, Shengyao Kuang & Yuancheng Zhou

  3. Key , Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China

    Ling Zhu & Zhiwen Xu

  4. Livestock and Poultry Biological Products Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu, China

    Yuancheng Zhou

  5. Livestock and Poultry Biological Products Key Laboratory of Sichuan Province, Animtech Bioengineering Co. Ltd, Chengdu, China

    Yuancheng Zhou

Authors
  1. Jun Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ling Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Huidan Deng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fengqing Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Lei Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xiangang Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Wenqi Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Shengyao Kuang
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Shuwei Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Yuancheng Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Zhiwen Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

ZX, YZ and LZ conceived the project. JZ and HD designed the experiments. FL, LX and SL performed most of the experiments. XS, WY and SK, contributed materials and participated in discussion. JZ wrote the manuscript. ZX supervised the work and edited the final version of the manuscript, which was read and approved by all authors.

Corresponding authors

Correspondence to Yuancheng Zhou or Zhiwen Xu.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

The authors confirm that the ethical policies of the journal, as noted on the journal’s author guidelines page, have been adhered to and that the appropriate ethical review committee approval was received. Approval was received from the Sichuan Provincial Laboratory Animal Management Committee (license no. SYXK (chuan) 2019-187). The “Guidelines for Experimental Animals” of the Ministry of Science and Technology (Beijing, China) were followed.

Additional information

Handling Editor: Jens H. Kuhn.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 10424 KB)

Supplementary file2 (FAS 15 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhao, J., Zhu, L., Deng, H. et al. Genetic characterization of a novel porcine reproductive and respiratory syndrome virus type I strain from southwest China. Arch Virol 166, 1769–1773 (2021). https://doi.org/10.1007/s00705-021-04998-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-021-04998-z

Search

Navigation