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First report of a novel partitivirus from the phytopathogenic fungus Fusarium cerealis in China

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Abstract

To our knowledge, no mycoviruses have been reported in Fusarium cerealis. Here, we describe a novel double-stranded RNA (dsRNA) virus, Fusarium cerealis partitivirus 1 (FcPV1), isolated from F. cerealis strain HN30 from Henan Province, China. The FcPV1 genome consists of two dsRNA segments, 1732 bp (dsRNA1) and 1361 bp (dsRNA2) in length, each containing a single open reading frame potentially encoding a 61.0-kDa protein and a 42.0-kDa protein, respectively. dsRNA1 encodes a putative RNA-dependent RNA polymerase (RdRp), while the dsRNA2 product has no significant similarity to any other capsid proteins (CPs) in the GenBank databases other than limited similarity to hypothetical “capsid” proteins of a few partitiviruses. Sequence alignments and phylogenetic analysis showed that FcPV1 is related to members of the newly proposed genus “Zetapartitivirus” in the family Partitiviridae.

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Funding

This work was supported by the National Key R&D Program of China (2018YFD0200500).

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Author notes

  1. Irfan Ahmed and Pengfei Li contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China

    Irfan Ahmed, Pengfei Li, Lihang Zhang, Pallab Bhattacharjee, Lihua Guo & Shuangchao Wang

  2. Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural University, Guangzhou, China

    Pengfei Li

  3. School of International Education, Beijing University of Chemical Technology, Beijing, China

    Xinwei Jiang

Authors
  1. Irfan Ahmed
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  2. Pengfei Li
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  3. Lihang Zhang
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  4. Xinwei Jiang
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  5. Pallab Bhattacharjee
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  6. Lihua Guo
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  7. Shuangchao Wang
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Correspondence to Shuangchao Wang.

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Fig. S1 (A)

Multiple alignment of the sequences of the conserved motifs in hypothetical proteins of FcPV1 and other homologous dsRNA viruses. Full names and accession numbers of selected viruses are as follows: AfPV1, Aspergillus flavus partitivirus 1 (QDE53635.1); BdV1, Botryosphaeria dothidea virus 1 (AIE47695.1); CaPV1, Colletotrichum acutatum partitivirus (AGL42313.1); CgRV1, Colletotrichum gloeosporioides RNA virus 1 (QED88096.1); PmPV1, Phoma matteucciicola partitivirus 1 (QDK65070.1); UvPV1, Ustilaginoidea virens partitivirus 1 (AGO04404.1). (B) Phylogenetic analysis based on protein sequences encoded by dsRNA2 of FcPV1 and other members of the family Partitiviridae. Bootstrap values higher than 60% are shown. The neighbor-joining (NJ) tree was constructed in MEGA 7.0 with 1000 bootstrap replicates. FcPV1 is indicated by a red circle (TIFF 7572 kb)

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Ahmed, I., Li, P., Zhang, L. et al. First report of a novel partitivirus from the phytopathogenic fungus Fusarium cerealis in China. Arch Virol 165, 2979–2983 (2020). https://doi.org/10.1007/s00705-020-04802-4

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  • DOI: https://doi.org/10.1007/s00705-020-04802-4

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