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Molecular characterization of a divergent strain of calla lily chlorotic spot virus infecting celtuce (Lactuca sativa var. augustana) in China

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Abstract

Through sequencing and assembly of small RNAs, an orthotospovirus was identified from a celtuce plant (Lactuca sativa var. augustana) showing vein clearing and chlorotic spots in the Zhejiang province of China. The S, M, and L RNAs of this orthotospovirus were determined to be 3146, 4734, and 8934 nt, respectively, and shared 30.4-72.5%, 43.4-80.8%, and 29.84-82.9% nucleotide sequence identities with that of known orthotospoviruses. The full length nucleoprotein (N) of this orthotospovirus shared highest amino acid sequence identity (90.25%) with that of calla lily chlorotic spot virus isolated from calla lily (CCSV-calla) [China: Taiwan: 2001] and tobacco (CCSV-LJ1) [China: Lijiang: 2014]. Phylogenetic analyses showed that this orthotospovirus is phylogenetically associated with CCSV isolates and clustered with CCSV, tomato zonate spot virus (TZSV), and tomato necrotic spot-associated virus (TNSaV) in a separate sub-branch. These results suggest that this orthotospovirus is a divergent isolate of CCSV and was thus named CCSV-Cel [China: Zhejiang: 2017].

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Acknowledgements

The authors would like to thank Dr. Zhongkai Zhang and Dr. Jiahong Dong from the Yunnan Academy of Agricultural Sciences for their valuable suggestions to this manuscript.

Funding

This study was financial funded by the National Natural Science Foundation of China (Grant no: 31671998).

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Authors and Affiliations

  1. College of Agriculture, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Xiaodong Wu, Xiaoyun Wu, Wenbin Li & Xiaofei Cheng

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  1. Xiaodong Wu
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  2. Xiaoyun Wu
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  3. Wenbin Li
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  4. Xiaofei Cheng
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Correspondence to Xiaofei Cheng.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: Ralf Georg Dietzgen.

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Wu, X., Wu, X., Li, W. et al. Molecular characterization of a divergent strain of calla lily chlorotic spot virus infecting celtuce (Lactuca sativa var. augustana) in China. Arch Virol 163, 1375–1378 (2018). https://doi.org/10.1007/s00705-018-3743-8

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  • DOI: https://doi.org/10.1007/s00705-018-3743-8

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