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Molecular characterization of a novel fusarivirus infecting the plant-pathogenic fungus Neofusicoccum luteum

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Abstract

Double-stranded RNAs from an isolate of Neofusicoccum luteum collected from grapevines were analyzed by high-throughput sequencing. Contig annotations revealed the presence of a potential novel virus belonging to the newly proposed family Fusariviridae. Completion of the viral genome sequence was performed. The genome is 6,244 nucleotide long, excluding the poly(A) tail and contains two putative open reading frames (ORFs). The first one encodes a large polypeptide of 1,552 amino acids (aa) with conserved RNA-dependent RNA polymerase and helicase domains typical of viral replicases. The second ORF encodes a putative 475-aa-long polypeptide showing weak homology to the corresponding ORF of Macrophomina phaseolina single-stranded RNA virus 1, for which no function is known so far. Phylogenetic analyses indicated that this virus should be considered a novel mycovirus belonging to the proposed family Fusariviridae, for which the name “Neofusicoccum luteum fusarivirus 1” (NlFV1) is proposed.

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Acknowledgements

The authors thank the Platform Genotoul (INRA, Toulouse, France) for the Illumina sequencing.

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Authors and Affiliations

  1. INRA, UMR 1332 Biologie du Fruit et Pathologie, University of Bordeaux, 71 Avenue Edouard Bourlaux, CS 20032, 33882, Villenave d’Ornon Cedex, France

    Armelle Marais, Chantal Faure, Sébastien Theil & Thierry Candresse

  2. INRA, UMR 1065 Santé et Agroécologie du Vignoble, Bordeaux Sciences Agro, 71 Avenue Edouard Bourlaux, CS 20032, 33882, Villenave d’Ornon Cedex, France

    Aurélia Nivault, Gwenaelle Comont & Marie-France Corio-Costet

Authors
  1. Armelle Marais
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  2. Aurélia Nivault
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  3. Chantal Faure
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  4. Gwenaelle Comont
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  5. Sébastien Theil
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  6. Thierry Candresse
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  7. Marie-France Corio-Costet
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Corresponding author

Correspondence to Armelle Marais.

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All authors declare no conflict of interest.

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This article does not contain any studies with animals or human participants performed by any of the authors.

Additional information

Handling Editor: Robert H.A. Coutts.

The nucleotide sequence reported in this study has been deposited in GenBank under the accession number KY906213.

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Marais, A., Nivault, A., Faure, C. et al. Molecular characterization of a novel fusarivirus infecting the plant-pathogenic fungus Neofusicoccum luteum . Arch Virol 163, 559–562 (2018). https://doi.org/10.1007/s00705-017-3620-x

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  • DOI: https://doi.org/10.1007/s00705-017-3620-x

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