Abstract
Foot-and-mouth disease (FMD), caused by foot-and-mouth disease virus (FMDV), is a highly contagious infectious disease that affects domestic and wild cloven-hoofed animals worldwide. VP2 is a structural protein of FMDV. In this study, a potent FMDV serotype-independent monoclonal antibody (MAb) 3D9 was generated. Screening of a phage-displayed random 12-peptide library revealed that MAb 3D9 bound to phages displaying a consensus motif GVYxxAYxW that is highly homologous to the 89GVYxxxxxxxAYxxxxW105 motif in the FMDV VP2 protein. Importantly, this conformational epitope was highly conserved among all seven serotypes of FMDV analyzed in sequence alignments. To further verify the authentic epitope recognized by MAb 3D9, a FMDV O/YS/CHA/05 mutant virus V90A was generated using a reverse genetics system. The results revealed that Val90 was an important residue for MAb 3D9 binding within this conformational epitope. Thus, we finely mapped a conserved conformational epitope onto the FMDV VP2 protein. These results could be applied in the development of epitope-based vaccines and suitable MAb-based diagnostic methods for various FMDV serotype-independent tests.
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Acknowledgements
This work was supported by a grant from the Key Project of Heilongjiang Provincial Science and Technology Program (No. GA06B202).
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Animal welfare and experimental procedures were carried out strictly according to the American Physiological Society’s Guiding Principles for the Care and Use of Animals and approved by the Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences. The animal ethics committee approval number is SYXK (Hei) 2012-2067.
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The authors declare no conflict of interest.
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Mice were acclimatized for one week prior to the beginning of the experiment, and were bred in clean and spacious animal facilities, and handled humanely according to the rules described by the Animal Ethics Procedures and Guidelines of the People’s Republic of China and the Animal Ethics Committee of HVRI, Chinese Academy of Agricultural Sciences [SYXK (Hei) 2012-2067]. The mice were euthanized by exsanguination under deep anesthesia (intramuscular injection of chlorpromazine at 2-6 mg/kg) at the end of the experiment.
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Liu, W., Yang, B., Wang, M. et al. Identification of a conserved conformational epitope in the VP2 protein of foot-and-mouth disease virus. Arch Virol 162, 1877–1885 (2017). https://doi.org/10.1007/s00705-017-3304-6
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DOI: https://doi.org/10.1007/s00705-017-3304-6