Abstract
Herpesviruses (HVs) have a wide range of hosts in the animal kingdom. The result of infection with HVs can vary from asymptomatic to fatal diseases depending on subtype, strain, and host. To date, little is known about HVs naturally circulating in wildlife species and the impact of these viruses on other species. In our study, we used genetic and comparative approaches to increase our understanding of circulating HVs in Canadian wildlife. Using nested polymerase chain reaction targeting a conserved region of the HV DNA polymerase gene, we analyzed material derived from wildlife of western and northern Canada collected between February 2009 and Sept 2014. For classification of new virus sequences, we compared our viral sequences with published sequences in GenBank to identify conserved residues and motifs that are unique to each subfamily, alongside phylogenetic analysis. All alphaherpesviruses shared a conserved tryptophan (W856) and tyrosine (Y880), betaherpesviruses all shared a serine (S836), and gammaherpesviruses had a conserved glutamic acid (E835). Most of our wildlife HV sequences grouped together with HVs from taxonomically related host species. From Martes americana, we detected previously uncharacterized alpha- and beta-herpesviruses.
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References
McGeoch DJ, Gatherer D (2005) Integrating reptilian herpesviruses into the family Herpesviridae. J Virol 79:725–731
McGeoch DJ, Rixon FJ, Davison AJ (2006) Topics in herpesvirus genomics and evolution. Virus Res 117:90–104
Wang N, Baldi PF, Gaut BS (2007) Phylogenetic analysis, genome evolution and the rate of gene gain in the Herpesviridae. Mol Phylogenet Evol 43(3):1066–1075
Blake N (2010) Immune evasion by gammaherpesvirus genome maintenance proteins. J Gen Virol 91(4):829–846
McGeoch DJ, Cook S, Dolan A, Jamieson FE, Telford EA (1995) Molecular phylogeny and evolutionary timescale for the family of mammalian herpesviruses. J Mol Biol 247:443–458
McGeoch DJ, Dolan A, Ralph AC (2000) Toward a comprehensive phylogeny for mammalian and avian herpesviruses. J Virol 74:10401–10406
Wald A, Corey L (2007) Persistence in the population: epidemiology, transmission. In: Arvin A et al (ed) Human herpesviruses: biology, therapy, and immunoprophylaxis. Cambridge University Press, Cambridge, ch 36
Lankester F, Lugelo A, Kazwala R, Keyyu J, Cleaveland S, Yoder J (2015) The economic impact of malignant catarrhal fever on pastoralist livelihoods. PLoS One 10(1):e0116059
Mlilo D, Mhlanga M, Mwembe R, Sisito G, Moyo B, Sibanda B (2015) The epidemiology of malignant catarrhal fever (MCF) and contribution to cattle losses in farms around Rhodes Matopos National Park, Zimbabwe. Trop Anim Health Prod 47(5):989–994
Tessaro SV, Deregt D, Dzus E, Rohner C, Smith K, Gaboury T (2005) Herpesvirus infection in woodland caribou in Alberta, Canada. J Wild Dis 41(4):803–805
Garver KA, Al-Hussinee L, Hawley LM, Schroeder T, Edes S, LePage V et al (2010) Mass mortality associated with koi herpesvirus in wild common carp in Canada. J Wild Dis 46(4):1242–1251
Himworth CG, Haulena M, Lambourn DM, Gaydos JK, Huggins J, Calambokidis J et al (2010) Pathology and epidemiology of Phocid herpesvirus-1 in wild and rehabilitating harbor seals (Phoca vitulina richardsi) in the northeastern Pacific. J Wild Dis 46(3):1046–1051
Gailbreath K, Oaks L (2008) Herpesviral inclusion body disease in owls and falcons is caused by the pigeon herpesvirus (Columbid herpesvirus 1). J Wildl Dis 44:427–433
Rose N, Warren AL, Whiteside D, Bidulka J, Robinson JH, Illanes O, Brookfield C (2012) Columbid herpesvirus-1 mortality in great horned owls (Bubo virginianus) from Calgary, Alberta. Can Vet J 53:265–268
Brown M, Moore L, McMahon B, Powell D, LaBute M, Hyman JM et al (2015) Constructing rigorous and broad biosurveillance networks for detecting emerging zoonotic outbreaks. PLoS One 10(5):e0124037
Vandevanter DR, Warrener P, Bennett L, Schultz ER, Coulter S, Garber RL et al (1996) Detection and analysis of diverse herpesviral species by consensus primer PCR. J Clin Microbiol 34(7):1666–1671
Bennett N, Götte M (2013) Utility of bacteriophage RB69 polymerase gp43 as a surrogate enzyme for herpesvirus orthologs. Viruses 5:54–86
Marchler-Bauer A, Derbyshire MK, Gonzales NR, Lu S, Chitsaz F, Geer LY et al (2015) CDD: NCBI’s conserved domain database. Nucleic Acids Res 28(43):D222. doi:10.1093/nar/gku1221
Ye L-B, Huang E (1993) In vitro expression of the human cytomegalovirus DNApolymerase gene: Effects of sequence alterations on enzyme activity. J. Virol 67:6339–6347
Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S et al (2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinf 28(12):1647–1649
NCBI (2013) National Center for Biotechnology Information BLAST home. http://blast.ncbi.nlm.nih.gov/Blast.cgi
Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high 180 throughput. Nucleic Acids Res 32(5):1792–1797
Stamatakis A (2014) RAxML Version 8: A tool for Phylogenetic Analysis and Post-Analysis of Large Phylogenies. Bioinf 30(9):1312–1313
Morariu VI, Srinivasan BV, Raykar VC, Duraiswami R, Davis LS (2008) Automatic online tuning for fast Gaussian summation. Adv Neur Inf Proc Sys (NIPS). http://sourceforge.net/projects/figtree/
Li H, Gailbreath K, Flach EJ, Taus NS, Cooley J, Keller J et al (2005) A novel subgroup of rhadinoviruses in ruminants. J Gen Virol 86:3021–3026
das Neves CG, Ihlebaek HM, Skjerve E, Hemmingsen W, Li H, Tryland M (2013) Gammaherpesvirus infection in semi-domesticated reindeer (Rangifer tarandus tarandus): a cross-sectional, serologic study in northern Norway. J Wild Dis 49(2):261–269
COSEWIC (2011) Designatable units for caribou (Rangifer tarandus) in Canada. Committee on the Status of Endangered Wildlife in Canada: Ottawa, p 88. http://www.cosewic.gc.ca/eng/sct12/COSEWIC_Caribou_DU_Report_23Dec2011.pdf
Yannic G, Pellissier L, Ortego J, Lecomte N, Couturier S, Cuyler C (2014) Genetic diversity in caribou linked to past and future climate change. Nat Clim Change 4:132–137
Levi T, Wilmers CC (2012) Wolves-coyotes-foxes: a cascade among carnivores. Ecology 93(4):921–929
Broquet T, Johnson CA, Petit E, Thompson I, Burel F, Fryxell JM (2006) Dispersal and genetic structure in the American marten, Martes americana. Mol Ecol 15(6):1689–1697
Larder BA, Kemp SD, Darby G (1987) Related functional domains in virus DNA polymerases. EMBO J 6(1):169–175
Plumb JA, Wright LD, Jones VL (1973) Survival of channel catfish virus in chilled, frozen, and decomposing channel catfish. Progress Fish Culturist 35:170–172
Acknowledgements
We would like to thank the members of the Canadian Cooperative Wildlife Health Centre and the pathology department at the University of Calgary Spy Hill Campus for their help and expertise in animal sample collection during necropsy. Caribou samples were collected through CARMA project; some marten samples were collected by the youth of the Sahtú region as part of an NSERC PromoScience outreach program. Finally, Alasdair Veitch, Ale Massolo, and Cynthia Kashivakura participated largely in this project.
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This study was funded by University Research Grants Committee (URGC) Seed Grant Program, University of Calgary, Alberta, Canada.
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Dalton, C.S., van de Rakt, K., Fahlman, Å. et al. Discovery of herpesviruses in Canadian wildlife. Arch Virol 162, 449–456 (2017). https://doi.org/10.1007/s00705-016-3126-y
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DOI: https://doi.org/10.1007/s00705-016-3126-y