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Complete genome sequence and evolution analysis of Eimeria stiedai RNA virus 1, a novel member of the family Totiviridae

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Abstract

Eimeria stiedai (E. stiedai) is a coccidian that infects the liver of the domestic rabbit and may cause severe hepatic coccidiosis. Virus-like particles in E. stiedai were discovered by Revets et al. However, the complete genome sequence of the E. stiedai virus has yet to be determined. A novel virus was isolated from E. stiedai in the present study. The complete genome sequence of the E. stiedai virus was 6219 bp in length and contained two open reading frames (ORFs) with a tetranucleotide overlap (AUGA). ORF1 (2400 bp) encoded a putative coat protein of 799 amino acids (86.471 kDa) that exhibited a high level of amino acid sequence similarity to that of Eimeria tenella (E. tenella) RNA virus 1 (EtRV1; 43 % identity, NC_026140), whereas ORF2 (3303 bp) encoded a putative RNA-dependent RNA polymerase (RdRp) of 1100 amino acids (118.850 kDa) that exhibited a high level of amino acid sequence similarity to that of the E. tenella RNA virus 1 (EtRV1; 51 % identity, NC_026140). Phylogenetic analysis revealed that the E. stiedai virus was a new member of the family Totiviridae. The sequence data provided sufficient information for classification of eimeriaviruses.

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    Authors and Affiliations

    1. College of Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun, 130062, China

      Caiyan Xin, Bin Wu, Jianhua Li, Pengtao Gong, Ju Yang, He Li, Xuepeng Cai & Xichen Zhang

    2. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China

      Caiyan Xin & Xuepeng Cai

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    1. Caiyan Xin
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    2. Bin Wu
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    3. Jianhua Li
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    6. He Li
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    7. Xuepeng Cai
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    8. Xichen Zhang
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    Correspondence to Xuepeng Cai or Xichen Zhang.

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    This work was supported by the National Key Technology R&D Program (#2015BAI07B02), the National Natural Science Foundation of China (Nos. 31272550, 31072123 and 31372425), the Program for New Century Excellent Talents in University and the Science and Technology Department of Jilin Province (20070135).

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    The authors declare that they have no conflict of interest.

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    All animal experiments were approved by the Ethics Committee of Jilin University.

    Additional information

    C. Xin, B. Wu and J. Li contributed equally to this study.

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    Xin, C., Wu, B., Li, J. et al. Complete genome sequence and evolution analysis of Eimeria stiedai RNA virus 1, a novel member of the family Totiviridae . Arch Virol 161, 3571–3576 (2016). https://doi.org/10.1007/s00705-016-3020-7

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    • DOI: https://doi.org/10.1007/s00705-016-3020-7

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