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Molecular characterization of infectious bronchitis viruses isolated from broiler chicken farms in Iran, 2014-2015

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Abstract

Infectious bronchitis (IB) is a viral avian disease with economic importance in the world, including Iran. S1 gene sequencing has been used for molecular epidemiological studies and genotypic characterization of infectious bronchitis virus (IBV). A total of 118 IBV isolates were obtained from tissue samples from chickens with clinically suspected IB from Iranian broiler farms (eight provinces, 200 samples). The isolates were confirmed by real-time polymerase chain reaction (PCR) and characterized by sequencing the spike glycoprotein gene. The isolates formed six distinct phylogenetic groups (IS/1494/06 [Var2] like, 4/91-like, IS/720-like, QX-like, IR-1 and Mass-like) that were related to variants isolated in the region. The most frequently detected viruses were of the Var2-like (IS/1494/06-like) genotype, with an overall prevalence of 34 %. Twenty-one percent of the isolates formed a cluster together with the 4/91 IBV type, 10 % were of the QX genotype, and 8 % were of the IS/720 genotype. In addition, 4 % and 3 % of the isolates belonged to the Massachusetts and IR-1 genotype, respectively. For the first time, we have isolated and characterized IBV variants from broiler farms in different provinces of Iran. This study demonstrates a constant evolution of IBV in Iran, demonstrating the need for continuous monitoring and development of new vaccines based on indigenous viruses.

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Acknowledgments

The Research Council, University of Tehran (Grant No. 28692/6/1) and the Iranian Veterinary Organization (Grant No. 22/39007) financially supported this project. The authors gratefully acknowledge Dr. Abdolahi and Mr. Behrooz Asadi for their extensive technical supports.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IRAN

    Hamideh Najafi, Arash Ghalyanchi Langeroudi & Omid Madadgar

  2. Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute, Karaj, IRAN

    Masoud Hashemzadeh

  3. Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, IRAN

    Vahid Karimi

  4. Iranian veterinary Organization, Tehran, IRAN

    Seyed Ali Ghafouri, Hossein Maghsoudlo & Reza Khaltabadi Farahani

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  1. Hamideh Najafi
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  2. Arash Ghalyanchi Langeroudi
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Correspondence to Arash Ghalyanchi Langeroudi.

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Najafi, H., Langeroudi, A.G., Hashemzadeh, M. et al. Molecular characterization of infectious bronchitis viruses isolated from broiler chicken farms in Iran, 2014-2015. Arch Virol 161, 53–62 (2016). https://doi.org/10.1007/s00705-015-2636-3

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  • DOI: https://doi.org/10.1007/s00705-015-2636-3

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