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Identification and genetic characterization of two porcine astroviruses from domestic piglets in China

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Abstract

Porcine astrovirus are divided into five genotypes. In this study, we identified two porcine astroviruses (AstV-LL-1 and AstV-LL-2) by using sequence-independent single-primer amplification (SISPA) on faecal specimens of healthy domestic piglets younger than 15 days. The detection rate for both was 2.82 % (14/497). AstV-LLs were then sequenced and characterised. Phylogenetic analysis revealed that they have the characteristics of porcine astrovirus (PastV) 2 and 5 and have some unique genetic features. Our findings show that the two astroviruses are novel lineages of PAstV2 and 5. The findings may be helpful in evaluating the ecology and evolution of astroviruses in pigs.

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Acknowledgments

We thank all of the staff members of the LuLong CDC, China, who helped to obtain the porcine stools. This work was supported by a fund for China Mega-Project for Infectious Disease (2011ZX10004-001).

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    Authors and Affiliations

    1. National Institute for Viral Disease Control and Prevention, China CDC, 100 Ying-Xin St., Xuan-Wu District, Beijing, 100052, China

      Jin-song Li, Mao-zhong Li, Li-shu Zheng, Na Liu, Dan-di Li & Zhao-jun Duan

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    1. Jin-song Li
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    2. Mao-zhong Li
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    3. Li-shu Zheng
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    4. Na Liu
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    5. Dan-di Li
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    6. Zhao-jun Duan
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    Correspondence to Zhao-jun Duan.

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    The authors declare that they have no conflicts of interest.

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    J. Li, M. Li and L. Zheng contributed equally to the manuscript.

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    Li, Js., Li, Mz., Zheng, Ls. et al. Identification and genetic characterization of two porcine astroviruses from domestic piglets in China. Arch Virol 160, 3079–3084 (2015). https://doi.org/10.1007/s00705-015-2569-x

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    • DOI: https://doi.org/10.1007/s00705-015-2569-x

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