Abstract
The genome of tetterwort vein chlorosis virus (TVCV) from South Korea has been completely sequenced. Its genomic organization resembles those of other criniviruses, with several new features, indicating that TVCV is a member of a new species in the genus Crinivirus, family Closteroviridae. RNA1 contains 8467 nucleotides, with at least four opening reading frames (ORFs). ORF1a encodes a protein with predicted papain-like protease, methyltransferase, and helicase activities. ORF1b encodes a putative RNA-dependent RNA polymerase that is apparently expressed through a +1 ribosomal frameshift. RNA2 contains 8113 nucleotides encoding at least nine proteins, similar to most crinivirus RNA2s. The 3′ untranslated regions of the bipartite RNA genome share 82.1 % nucleotide sequence identity.
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Acknowledgments
This work was performed with the support of the Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ01007703 and Project No. PJ01112803), Rural Development Administration, Republic of Korea.
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F. Zhao and R. H. Yoo contributed equally to this study.
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Zhao, F., Yoo, R.H., Lim, S. et al. Nucleotide sequence and genome organization of a new proposed crinivirus, tetterwort vein chlorosis virus. Arch Virol 160, 2899–2902 (2015). https://doi.org/10.1007/s00705-015-2552-6
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DOI: https://doi.org/10.1007/s00705-015-2552-6