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Detection of a divergent variant of grapevine virus F by next-generation sequencing

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Abstract

The complete genome sequence of a South African isolate of grapevine virus F (GVF) is presented. It was first detected by metagenomic next-generation sequencing of field samples and validated through direct Sanger sequencing. The genome sequence of GVF isolate V5 consists of 7539 nucleotides and contains a poly(A) tail. It has a typical vitivirus genome arrangement that comprises five open reading frames (ORFs), which share only 88.96 % nucleotide sequence identity with the existing complete GVF genome sequence (JX105428).

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Acknowledgments

The financial assistance of Winetech and the Technology and Human Resources for Industry Programmes towards this research is hereby acknowledged. This work is based on the research supported in part by the NRF (UID 84535). The Grantholder acknowledges that opinions, findings and conclusions or recommendations expressed in any publication generated by NRF-supported research are those of the authors, and that the NRF accepts no liability whatsoever in this regard.

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Authors and Affiliations

  1. Department of Genetics, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa

    Nicholas Molenaar, Johan T. Burger & Hans J. Maree

  2. ARC Infruitec-Nietvoorbij (The Fruit, Vine and Wine Institute of the Agricultural Research Council), Private Bag X5026, Stellenbosch, 7599, South Africa

    Hans J. Maree

Authors
  1. Nicholas Molenaar
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  2. Johan T. Burger
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  3. Hans J. Maree
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Corresponding author

Correspondence to Hans J. Maree.

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Molenaar, N., Burger, J.T. & Maree, H.J. Detection of a divergent variant of grapevine virus F by next-generation sequencing. Arch Virol 160, 2125–2127 (2015). https://doi.org/10.1007/s00705-015-2466-3

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  • DOI: https://doi.org/10.1007/s00705-015-2466-3

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