Skip to main content
SpringerLink
Log in

Serological report of pandemic and seasonal human influenza virus infection in dogs in southern China

  • Original Article
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

From January to July 2012, we looked for evidence of subclinical A (H1N1) pdm09 and seasonal human influenza viruses infections in healthy dogs in China. Sera from a total of 1920 dogs were collected from Guangdong, Guangxi, Fujian and Jiangxi provinces. We also examined archived sera from 66 dogs and cats that were collected during 2008 from these provinces. Using hemagglutination inhibition (HI) and microneutralization (MN) assays, we found that only the dogs sampled in 2012 had elevated antibodies (≥1:32) against A(H1N1)pdm09 virus and seasonal human influenza viruses: Of the 1920 dog sera, 20.5 % (n = 393) had elevated antibodies against influenza A(H1N1) pdm09 by the HI assay, 1.1 % (n = 22), and 4.7 % (n = 91) of the 1920 dogs sera had elevated antibodies against human seasonal H1N1 influenza virus and human seasonal H3N2 influenza virus by the HI assay. Compared with dogs that were raised on farms, dogs that were raised as pets were more likely to have elevated antibodies against A(H1N1)pdm09 and seasonal human influenza viruses. Seropositivity was highest among pet dogs, which likely had more diverse and frequent exposures to humans than farm dogs. These findings will help us better understand which influenza A viruses are present in dogs and will contribute to the prevention and control of influenza A virus. Moreover, further in-depth study is necessary for us to understand what roles dogs play in the ecology of influenza A.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Su S, Qi W, Chen J, Zhu W, Huang Z, Xie J, Zhang G (2013) Seroepidemiological evidence of avian influenza A virus transmission to pigs in southern China. J Clin Microbiol 51:601–602

    Article  PubMed  PubMed Central  Google Scholar 

  2. Storms AD, Van Kerkhove MD, Azziz-Baumgartner E, Lee WK, Widdowson MA et al (2013) Worldwide transmission and seasonal variation of pandemic influenza A(H1N1)2009 virus activity during the 2009-2010 pandemic. Influenza Other Respir Viruses 7:1328–1335

    Article  PubMed  Google Scholar 

  3. Cao B, Li XW, Mao Y, Wang J, Lu HZ et al (2009) Clinical features of the initial cases of 2009 pandemic influenza A (H1N1) virus infection in China. N Engl J Med 361:2507–2517

    Article  PubMed  CAS  Google Scholar 

  4. Berhane Y, Ojkic D, Neufeld J, Leith M, Hisanaga T et al (2010) Molecular characterization of pandemic H1N1 influenza viruses isolated from turkeys and pathogenicity of a human pH1N1 isolate in turkeys. Avian Dis 54:1275–1285

    Article  PubMed  Google Scholar 

  5. Damiani AM, Kalthoff D, Beer M, Muller E, Osterrieder N (2012) Serological survey in dogs and cats for influenza A(H1N1)pdm09 in Germany. Zoonoses Public Health 59:549–552

    Article  PubMed  CAS  Google Scholar 

  6. Dundon WG, De Benedictis P, Viale E, Capua I (2010) Serologic evidence of pandemic (H1N1) 2009 infection in dogs, Italy. Emerg Infect Dis 16:2019–2021

    Article  PubMed  PubMed Central  Google Scholar 

  7. Li S, Shi Z, Jiao P, Zhang G, Zhong Z et al (2010) Avian-origin H3N2 canine influenza A viruses in Southern China. Infect Genet Evol 10:1286–1288

    Article  PubMed  PubMed Central  Google Scholar 

  8. Gray GC, Bender JB, Bridges CB, Daly RF, Krueger WS et al (2012) Influenza A (H1N1) pdm09 virus among healthy show pigs, United States. Emerg Infect Dis 18:1519

    Article  PubMed  PubMed Central  Google Scholar 

  9. Lin D, Sun S, Du L, Ma J, Fan L et al (2012) Natural and experimental infection of dogs with pandemic H1N1/2009 influenza virus. J Gen Virol 93:119–123

    Article  PubMed  CAS  Google Scholar 

  10. McCullers JA, Van De Velde LA, Schultz RD, Mitchell CG, Halford CR et al (2011) Seroprevalence of seasonal and pandemic influenza A viruses in domestic cats. Arch Virol 156:117–120

    Article  PubMed  CAS  Google Scholar 

  11. Ramírez-Martínez LA, Contreras-Luna M, la Luz J, Manjarrez ME, Rosete DP et al (2013) Evidence of transmission and risk factors for influenza A virus in household dogs and their owners. Influ Other Respir Viruses 7:1292–1296

    Article  Google Scholar 

  12. Crawford PC, Dubovi EJ, Castleman WL, Stephenson I, Gibbs EP et al (2005) Transmission of equine influenza virus to dogs. Science 310:482–485

    Article  PubMed  CAS  Google Scholar 

  13. Song D, Kang B, Lee C, Jung K, Ha G et al (2008) Transmission of avian influenza virus (H3N2) to dogs. Emerg Infect Dis 14:741–746

    Article  PubMed  PubMed Central  Google Scholar 

  14. Chang CP, New AE, Taylor JF, Chiang HS (1976) Influenza virus isolations from dogs during a human epidemic in Taiwan. Int J Zoonoses 3:61–64

    PubMed  CAS  Google Scholar 

  15. de Jong JC, Palache AM, Beyer WE, Rimmelzwaan GF, Boon AC, Osterhaus AD (2003) Haemagglutination-inhibiting antibody to influenza virus. Dev Biol (Basel) 115:63–73

    Google Scholar 

  16. Eichelberger M, Golding H, Hess M, Weir J, Subbarao K, Luke CJ, Friede M, Wood D (2008) FDA/NIH/WHO public workshop on immune correlates of protection against influenza A viruses in support of pandemic vaccine development, Bethesda, Maryland, US, December 10–11, 2007. Vaccine 26:4299–4303

    Article  PubMed  Google Scholar 

  17. Lerdsamran H, Pittayawonganon C, Pooruk P, Mungaomklang A, Iamsirithaworn S, Thongcharoen P, Kositanont U, Auewarakul P, Chokephaibulkit K, Oota S, Pongkankham W, Silaporn P, Komolsiri S, Noisumdaeng P, Chotpitayasunondh T, Sangsajja C, Wiriyarat W, Louisirirotchanakul S, Puthavathana P (2011) Serological response to the 2009 pandemic influenza A (H1N1) virus for disease diagnosis and estimating the infection rate in Thai population. PloS ONE 6:e16164

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  18. Reed LJ, Muench H (1938) A simple method of estimating fifty per cent endpoints. Am J Epidemiol 27:493–497

    Google Scholar 

  19. Rowe T, Abernathy RA, Hu-Primmer J, Thompson WW, Lu X et al (1999) Detection of antibody to avian influenza A (H5N1) virus in human serum by using a combination of serologic assays. J Clin Microbiol 37:937–943

    PubMed  CAS  PubMed Central  Google Scholar 

  20. Sun Y, Shen Y, Zhang X, Wang Q, Liu L, Han X, Jiang B, Wang R, Sun H, Pu J, Lin D, Xia Z, Liu J (2014) A serological survey of canine H3N2, pandemic H1N1/09 and human seasonal H3N2 influenza viruses in dogs in China. Vet Microbiol 168(1):193–196

    Article  PubMed  Google Scholar 

  21. Lessler J, Reich NG, Cummings DA (2009) Outbreak of 2009 pandemic influenza A (H1N1) at a New York City school. N Engl J Med 361:2628–2636

    Article  PubMed  CAS  Google Scholar 

  22. Guan Y, Shortridge KF, Krauss S, Chin PS, Dyrting KC et al (2000) H9N2 influenza viruses possessing H5N1-like internal genomes continue to circulate in poultry in southeastern China. J Virol 74:9372–9380

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  23. Su S, Zhou P, Fu X et al (2014) Virological and epidemiological evidence of avian influenza virus infections among feral dogs in live poultry markets, China. A threat to human health? Clin Infect Dis. doi:10.1093/cid/ciu154

    Google Scholar 

  24. Su S, Qi W, Zhou P et al (2014) First evidence of H10N8 avian influenza virus infections among feral dogs in live poultry markets in Guangdong province, China. Clin Infect Dis. doi:10.1093/cid/ciu345

Download references

Acknowledgments

This work was supported by National Transgenic Project of China (2011ZX08011-004) and Basic Conditions for Science and Technology Projects of Lanzhou City (2012-2-71).

Author information

Authors and Affiliations

  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150001, People’s Republic of China

    Xin Yin, Dong-Hui Zhou & Ping Wei

  2. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, People’s Republic of China

    Fu-Rong Zhao, Dong-Hui Zhou & Hui-Yun Chang

Authors
  1. Xin Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fu-Rong Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dong-Hui Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ping Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hui-Yun Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Ping Wei or Hui-Yun Chang.

Additional information

X. Yin and F.-R. Zhao contributed equally to this study.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yin, X., Zhao, FR., Zhou, DH. et al. Serological report of pandemic and seasonal human influenza virus infection in dogs in southern China. Arch Virol 159, 2877–2882 (2014). https://doi.org/10.1007/s00705-014-2119-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-014-2119-y

Keywords

Search

Navigation